PUBLICATION

The zebrafish galectins Drgal1-L2 and Drgal3-L1 bind in vitro to the infectious hematopoietic necrosis virus (IHNV) glycoprotein and reduce viral adhesion to fish epithelial cells

Authors
Nita-Lazar, M., Mancini, J., Feng, C., González-Montalbán, N., Ravindran, C., Jackson, S., Heras-Sánchez, A.L., Giomarelli, B., Ahmed, H., Haslam, S.M., Wu, G., Dell, A., Ammayappan, A., Vakharia, V.N., Vasta, G.R.
ID
ZDB-PUB-151003-4
Date
2016
Source
Developmental and comparative immunology   55: 241-52 (Journal)
Registered Authors
Keywords
IHNV envelope glycoprotein, epithelial cells, galectin, infectious hematopoietic necrosis virus (IHNV), viral adhesion, zebrafish
MeSH Terms
  • Galectins/genetics
  • Galectins/metabolism*
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism*
  • Rhabdoviridae Infections/immunology*
  • Rhabdoviridae Infections/transmission
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Viral Envelope Proteins/metabolism*
  • Infectious hematopoietic necrosis virus/immunology*
  • Infectious hematopoietic necrosis virus/pathogenicity
  • Virus Attachment
  • Host-Pathogen Interactions
  • Animals
  • Epithelial Cells/physiology*
  • Epithelial Cells/virology
  • Cells, Cultured
  • Zebrafish/immunology*
  • Virulence
(all 19)
PubMed
26429411 Full text @ Dev. Comp. Immunol.
Abstract
The infectious hematopoietic necrosis virus (IHNV; Rhabdoviridae, Novirhabdovirus) infects teleost fish, such as salmon and trout, and is responsible for significant losses in the aquaculture industry and in wild fish populations. Although IHNV enters the host through the skin at the base of the fins, the viral adhesion and entry mechanisms are not fully understood. In recent years, evidence has accumulated in support of the key roles played by protein-carbohydrate interactions between host lectins secreted to the extracellular space and virion envelope glycoproteins in modulating viral adhesion and infectivity. In this study, we assessed in vitro the potential role(s) of zebrafish (Danio rerio) proto type galectin-1 (Drgal1-L2) and a chimera galectin-3 (Drgal3-L1) in IHNV adhesion to epithelial cells. Our results suggest that the extracellular Drgal1-L2 and Drgal3-L1 interact directly and in a carbohydrate-dependent manner with the IHNV glycosylated envelope and glycans on the epithelial cell surface, significantly reducing viral adhesion.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Nita-Lazar, M., Mancini, J., Feng, C., González-Montalbán, N., Ravindran, C., Jackson, S., Heras-Sánchez, A.L., Giomarelli, B., Ahmed, H., Haslam, S.M., Wu, G., Dell, A., Ammayappan, A., Vakharia, V.N., Vasta, G.R. (2016) The zebrafish galectins Drgal1-L2 and Drgal3-L1 bind in vitro to the infectious hematopoietic necrosis virus (IHNV) glycoprotein and reduce viral adhesion to fish epithelial cells. Developmental and comparative immunology. 55:241-52.