PUBLICATION
3D Light-Sheet Fluorescence Microscopy of Cranial Neurons and Vasculature during Zebrafish Embryogenesis
- Authors
- Park, O.K., Kwak, J., Jung, Y.J., Kim, Y.H., Hong, H.S., Hwang, B.J., Kwon, S.H., Kee, Y.
- ID
- ZDB-PUB-151003-3
- Date
- 2015
- Source
- Molecules and cells 38(11): 975-81 (Journal)
- Registered Authors
- Jung, Yoo Jung, Kee, Yun, Kwak, Jina
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/anatomy & histology
- Animals, Genetically Modified/embryology
- Blood Vessels/embryology
- Brain/blood supply
- Brain/cytology
- Brain/embryology*
- Embryo, Nonmammalian/blood supply
- Embryo, Nonmammalian/cytology
- Imaging, Three-Dimensional/methods*
- Microscopy, Fluorescence/methods*
- Models, Animal
- Neuroimaging/methods*
- Neurons/ultrastructure*
- Skull/blood supply
- Skull/embryology
- Zebrafish/anatomy & histology
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 26429501 Full text @ Mol. Cells
Citation
Park, O.K., Kwak, J., Jung, Y.J., Kim, Y.H., Hong, H.S., Hwang, B.J., Kwon, S.H., Kee, Y. (2015) 3D Light-Sheet Fluorescence Microscopy of Cranial Neurons and Vasculature during Zebrafish Embryogenesis. Molecules and cells. 38(11):975-81.
Abstract
Precise 3D spatial mapping of cells and their connections within living tissues is required to fully understand developmental processes and neural activities. Zebrafish embryos are relatively small and optically transparent, making them the vertebrate model of choice for live in vivo imaging. However, embryonic brains cannot be imaged in their entirety by confocal or two-photon microscopy due to limitations in optical range and scanning speed. Here, we use light-sheet fluorescence microscopy to overcome these limitations and image the entire head of live transgenic zebrafish embryos. We simultaneously imaged cranial neurons and blood vessels during embryogenesis, generating comprehensive 3D maps that provide insight into the coordinated morphogenesis of the nervous system and vasculature during early development. In addition, blood cells circulating through the entire head, vagal and cardiac vasculature were also visualized at high resolution in a 3D movie. These data provide the foundation for the construction of a complete 4D atlas of zebrafish embryogenesis and neural activity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping