PUBLICATION
Differential expression of CARMIL-family genes during zebrafish development
- Authors
- Stark, B.C., Cooper, J.A.
- ID
- ZDB-PUB-151002-2
- Date
- 2015
- Source
- Cytoskeleton (Hoboken, N.J.) 72(10): 534-41 (Journal)
- Registered Authors
- Cooper, John, Stark, Benjamin
- Keywords
- actin, capping protein, lrrc16a, lrrc16b, rltpr
- MeSH Terms
-
- Actin Capping Proteins/metabolism
- Actins/metabolism
- Amino Acid Sequence
- Animals
- Carrier Proteins/metabolism
- DNA, Complementary/genetics
- Gene Expression Regulation, Developmental*
- In Situ Hybridization
- Microfilament Proteins/genetics
- Microfilament Proteins/metabolism*
- Multigene Family
- Phylogeny
- Protein Domains
- Protein Isoforms/metabolism
- RNA/metabolism
- Zebrafish/genetics
- Zebrafish/growth & development*
- PubMed
- 26426389 Full text @ Cytoskeleton
Citation
Stark, B.C., Cooper, J.A. (2015) Differential expression of CARMIL-family genes during zebrafish development. Cytoskeleton (Hoboken, N.J.). 72(10):534-41.
Abstract
CARMILs are a conserved family of large multidomain proteins that regulate and target actin assembly by interacting with actin capping protein (CP). Vertebrates contain three highly conserved CARMIL isoforms encoded by three genes, whereas lower organisms contain only one isoform and gene. In order to investigate the functions of vertebrate CARMILs, we identified and characterized the three CARMIL genes in zebrafish (Danio rerio). We isolated and sequenced complete and partial cDNAs from embryos. The three genes display distinct spatial and temporal expression patterns during development. Sequence and phylogenetic analyses of cDNAs and predicted protein sequences reveal that the three zebrafish genes fall into the three conserved isoform groups previously defined for other vertebrates, which have isoform-specific and overlapping functions in human cultured cells. These results provide new tools and offer insight into understanding the role of the regulation of actin assembly dynamics during embryonic development and tissue morphogenesis. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping