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ZFIN ID: ZDB-PUB-150925-2
Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide
Liu, T., Lin, J., Li, Z., Lin, L., Shen, Y., Zhu, H., Qian, Y.
Date: 2015
Source: The Analyst   140(21): 7165-9 (Journal)
Registered Authors: Li, Zhen
Keywords: none
MeSH Terms:
  • Animals
  • Cyclohexanones/chemistry
  • Fluorescent Dyes/chemistry*
  • HeLa Cells
  • Humans
  • Hydrogen Sulfide/chemistry*
  • Microscopy, Confocal
  • Microscopy, Fluorescence*
  • Models, Chemical
  • Nitriles/chemistry*
  • Zebrafish
PubMed: 26401525 Full text @ Analyst
ABSTRACT
We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive monitoring of hydrogen sulfide based on a dicyanoisophorone platform. An excellent linear relationship of fluorescence intensity ratio (I637/I558) (R(2) = 0.9867) versus hydrogen sulfide concentration in the range of 1-12 μM was obtained. This probe exhibited a remarkable fluorescence response to hydrogen sulfide over other physiological thiols or biological species, which fluoresces in the red region with a large Stokes shift (172 nm). This probe was successfully utilized to monitor H2S under in vitro physiological conditions and for imaging H2S in living cells and living zebrafish in vivo.
ADDITIONAL INFORMATION No data available