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ZFIN ID: ZDB-PUB-150925-2
Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide
Liu, T., Lin, J., Li, Z., Lin, L., Shen, Y., Zhu, H., Qian, Y.
Date: 2015
Source: The Analyst   140(21): 7165-9 (Journal)
Registered Authors: Li, Zhen
Keywords: none
MeSH Terms:
  • Animals
  • Cyclohexanones/chemistry
  • Fluorescent Dyes/chemistry*
  • HeLa Cells
  • Humans
  • Hydrogen Sulfide/chemistry*
  • Microscopy, Confocal
  • Microscopy, Fluorescence*
  • Models, Chemical
  • Nitriles/chemistry*
  • Zebrafish
PubMed: 26401525 Full text @ Analyst
We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive monitoring of hydrogen sulfide based on a dicyanoisophorone platform. An excellent linear relationship of fluorescence intensity ratio (I637/I558) (R(2) = 0.9867) versus hydrogen sulfide concentration in the range of 1-12 μM was obtained. This probe exhibited a remarkable fluorescence response to hydrogen sulfide over other physiological thiols or biological species, which fluoresces in the red region with a large Stokes shift (172 nm). This probe was successfully utilized to monitor H2S under in vitro physiological conditions and for imaging H2S in living cells and living zebrafish in vivo.