PUBLICATION
Large-scale live imaging of adult neural stem cells in their endogenous niche
- Authors
- Dray, N., Bedu, S., Vuillemin, N., Alunni, A., Coolen, M., Krecsmarik, M., Supatto, W., Beaurepaire, E., Bally-Cuif, L.
- ID
- ZDB-PUB-150924-5
- Date
- 2015
- Source
- Development (Cambridge, England) 142(20): 3592-600 (Journal)
- Registered Authors
- Alunni, Alessandro, Bally-Cuif, Laure, Bedu, Sebastien, Coolen, Marion, Dray, Nicolas
- Keywords
- SHG/THG, Live imaging, Multiphoton microscopy, Neural stem cell, Pallium, Zebrafish
- MeSH Terms
-
- Animals
- Image Processing, Computer-Assisted/methods
- Cell Lineage
- Luminescent Proteins/metabolism
- Microscopy, Fluorescence
- Animals, Genetically Modified
- Green Fluorescent Proteins/metabolism
- Neural Stem Cells/cytology*
- Stem Cell Niche*
- Brain/metabolism*
- Cell Proliferation
- Transgenes
- Telencephalon
- Hydrogen-Ion Concentration
- Neuroimaging/methods*
- Zebrafish
- Temperature
- PubMed
- 26395477 Full text @ Development
Citation
Dray, N., Bedu, S., Vuillemin, N., Alunni, A., Coolen, M., Krecsmarik, M., Supatto, W., Beaurepaire, E., Bally-Cuif, L. (2015) Large-scale live imaging of adult neural stem cells in their endogenous niche. Development (Cambridge, England). 142(20):3592-600.
Abstract
Live imaging of adult neural stem cells (aNSCs) in vivo is a technical challenge in the vertebrate brain. Here, we achieve long-term imaging of the adult zebrafish telencephalic neurogenic niche and track a population of more than 1,000 aNSCs over weeks, by taking advantage of fish transparency at near-infrared wavelengths and of intrinsic multiphoton landmarks. This methodology enables us to describe the frequency, distribution and modes of aNSCs divisions across the entire germinal zone of the adult pallium, and to highlight regional differences in these parameters.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping