| ZFIN ID: ZDB-PUB-150917-10 |
Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins
Ju Shin, Y., Kyun Park, S., Jung Jung, Y., Na Kim, Y., Sung Kim, K., Kyu Park, O., Kwon, S.H., Ho Jeon, S., Trinh, l.e. .A., Fraser, S.E., Kee, Y., Joon Hwang, B.
| Date: | 2015 |
|---|---|
| Source: | Scientific Reports 5: 14269 (Journal) |
| Registered Authors: | Fraser, Scott E., Trinh, Le |
| Keywords: | none |
| MeSH Terms: |
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| PubMed: | 26373678 Full text @ Sci. Rep. |
Citation
Ju Shin, Y., Kyun Park, S., Jung Jung, Y., Na Kim, Y., Sung Kim, K., Kyu Park, O., Kwon, S.H., Ho Jeon, S., Trinh, l.e. .A., Fraser, S.E., Kee, Y., Joon Hwang, B. (2015) Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins. Scientific Reports. 5:14269.
ABSTRACT
Targeted protein degradation is a powerful tool in determining the function of specific proteins or protein complexes. We fused nanobodies to SPOP, an adaptor protein of the Cullin-RING E3 ubiquitin ligase complex, resulting in rapid ubiquitination and subsequent proteasome-dependent degradation of specific nuclear proteins in mammalian cells and zebrafish embryos. This approach is easily modifiable, as substrate specificity is conferred by an antibody domain that can be adapted to target virtually any protein.
ADDITIONAL INFORMATION