PUBLICATION
Micrometam C Protects against Oxidative Stress in Inflammation Models in Zebrafish and RAW264.7 Macrophages
- Authors
- Tang, H., Ge, H., Chen, Z.B., Luo, X.M., Su, F.J., Liang, Y.B., Li, Z.Y., Wu, J.G., Yang, Q., Zeng, L.J., Ma, Z.F.
- ID
- ZDB-PUB-150908-5
- Date
- 2015
- Source
- Marine drugs 13: 5593-5605 (Journal)
- Registered Authors
- Keywords
- NADPH oxidase, NF-κB, inflammatory, micrometam C, zebrafish
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents/chemistry*
- Anti-Inflammatory Agents/pharmacology*
- Antioxidants
- Cell Line
- Coumaric Acids/chemistry*
- Coumaric Acids/pharmacology*
- Gene Expression Regulation/drug effects
- Inflammation/drug therapy*
- Lipopolysaccharides/toxicity
- Macrophages
- Mice
- Molecular Structure
- NADPH Oxidases/metabolism
- NF-kappa B/genetics
- NF-kappa B/metabolism
- Oxidative Stress
- Respiratory Burst
- Tail
- Zebrafish
- PubMed
- 26343688 Full text @ Mar. Drugs
Citation
Tang, H., Ge, H., Chen, Z.B., Luo, X.M., Su, F.J., Liang, Y.B., Li, Z.Y., Wu, J.G., Yang, Q., Zeng, L.J., Ma, Z.F. (2015) Micrometam C Protects against Oxidative Stress in Inflammation Models in Zebrafish and RAW264.7 Macrophages. Marine drugs. 13:5593-5605.
Abstract
Micrometam C is a core of novel marine compound isolated from the mangrove associates Micromelum falcatum. In this study, we investigated the protective effects of micrometam C in inflammation models in the transgenic zebrafish line Tg (corola: eGFP) and RAW264.7 macrophages. We found that micrometam C significantly suppressed the migration of immune cells in tail-cutting-induced inflammation in transgenic zebrafish and reduced lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) in both zebrafish and macrophages. In addition, micrometam C also restored LPS-induced reduction of endogenous antioxidants, such as catalase (CAT), glutathione (GSH) and superoxide dismutase (SOD). The protective effects of micrometam C were in parallel to its inhibition of NADPH oxidase and nuclear factor-kappa-binding (NF-κB) activity. Thus, the present results demonstrate that micrometam C protects against LPS-induced inflammation possibly through its antioxidant property.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping