PUBLICATION
Enantioselective bioaccumulation of hexaconazole and its toxic effects in adult zebrafish (Danio rerio)
- Authors
- Wang, Y., Xu, L., Li, D., Teng, M., Zhang, R., Zhou, Z., Zhu, W.
- ID
- ZDB-PUB-150822-10
- Date
- 2015
- Source
- Chemosphere 138: 798-805 (Journal)
- Registered Authors
- Keywords
- Apoptosis, Enantioselective bioaccumulation, Hexaconazole, Oxidative stress
- MeSH Terms
-
- Animals
- Antioxidants/metabolism
- Apoptosis/drug effects
- Apoptosis/genetics
- Caspase 3/metabolism
- Caspase 9/metabolism
- Down-Regulation
- Environmental Monitoring/methods
- Female
- Gene Expression/drug effects
- Liver/drug effects*
- Liver/metabolism
- Male
- Molecular Structure
- Oxidative Stress/drug effects
- Oxidative Stress/genetics
- Stereoisomerism
- Triazoles/analysis
- Triazoles/chemistry
- Triazoles/pharmacokinetics*
- Triazoles/toxicity*
- Up-Regulation
- Water Pollutants, Chemical/analysis
- Water Pollutants, Chemical/chemistry
- Water Pollutants, Chemical/pharmacokinetics*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 26291761 Full text @ Chemosphere
Citation
Wang, Y., Xu, L., Li, D., Teng, M., Zhang, R., Zhou, Z., Zhu, W. (2015) Enantioselective bioaccumulation of hexaconazole and its toxic effects in adult zebrafish (Danio rerio). Chemosphere. 138:798-805.
Abstract
Little is known about the bioaccumulation and toxicity of hexaconazole (HEX) in spite of the fact that they are indispensable parts for a comprehensive assessment of its environmental behavior and toxic effects in organisms of freshwater ecosystems. In this study, adult zebrafish were used to study the enantioselective bioaccumulation of HEX and its effect endpoints in liver, including oxidative stress and the regulation of apoptosis-related gene expression. Significant enantioselective bioaccumulation was demonstrated when exposed to HEX of 100 and 200μgL(-)(1), finding that the (-)-enantiomer tended to accumulate in zebrafish more easily than (+)-enantiomer. Activities of antioxidant enzymes (SOD, CAT and GPx) and GSH content were all significantly decreased when zebrafish were exposed to 50 and 200μgL(-)(1) HEX for 21d. A series of genes of the apoptosis pathway were examined in groups treated with 50 and 200μgL(-)(1) HEX for 21d using real-time PCR. Significant up-regulation of p53, Puma, Apaf-1, caspase-3 and caspase-9 expression and down-regulation of Bcl-2/Bax expression ratio were proved. The overall results indicated that waterborne HEX was able to produce oxidative stress and induce apoptosis through the involvement of caspases in adult zebrafish. The above information will play a vital role in the integrated environmental risk assessment of HEX and make its toxic mechanism in fish clear.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping