PUBLICATION
Sebox regulates mesoderm formation in early amphibian embryos
- Authors
- Chen, G., Tan, R.B., Tao, Q.H.
- ID
- ZDB-PUB-150819-1
- Date
- 2015
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 244(11): 1415-26 (Journal)
- Registered Authors
- Keywords
- Mix-like genes, PAPC, Wnt11, Xenopus, non-canonical Wnt
- MeSH Terms
-
- Gene Expression Regulation, Developmental*
- Homeodomain Proteins/physiology*
- Genes, Homeobox/genetics
- Genes, Homeobox/physiology*
- Gastrula/physiology
- Wnt Proteins/metabolism
- Cadherins/genetics
- Cadherins/metabolism
- Cadherins/physiology*
- Gastrulation
- Animals
- Mesoderm/physiology*
- Xenopus Proteins/genetics
- Xenopus Proteins/physiology*
- Xenopus/embryology*
- In Situ Hybridization
- Gene Expression Profiling
- Ligands
- DNA, Complementary/metabolism
- Signal Transduction
- Activins/metabolism
- Nodal Protein/metabolism
- PubMed
- 26285158 Full text @ Dev. Dyn.
Citation
Chen, G., Tan, R.B., Tao, Q.H. (2015) Sebox regulates mesoderm formation in early amphibian embryos. Developmental Dynamics : an official publication of the American Association of Anatomists. 244(11):1415-26.
Abstract
Background Mix/Bix genes are important regulators of mesendoderm formation during vertebrate embryogenesis. Sebox, an additional member of this gene family, has been implicated in endoderm formation during early embryogenesis in zebrafish. However, it remains unclear whether Sebox plays a unique role in early Xenopus embryos.
Results In this study, we provide evidence that Sebox is uniquely required for the formation of mesoderm during early Xenopus embryogenesis. Sebox is dynamically expressed in the involuted mesoderm during gastrulation. It is activated by Nodal/Activin signaling and modulated by zygotic Wnt/β-catenin signaling. Overexpression of Sebox perturbs movements during convergent extension and inhibits the expression of mesodermal, but not endodermal, genes induced by Nodal/Activin signaling. Depletion of Sebox using a specific MO increases the expression of non-canonical wnt5a, wnt5b, and wnt11b. Depletion of Sebox also upregulates the expression of pcdh8.2, a paraxial mesoderm-specific protocadherin, in a Wnt11B-dependent manner. Sebox morphants display reduced development of the head and notochord.
Conclusions Our findings illustrate that Sebox, a unique member of the Mix/Bix gene family, functions downstream of Nodal/Activin signaling and is required for the proper expression of non-canonical Wnt ligands and the normal development of mesoderm in Xenopus. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping