PUBLICATION

Nerves Regulate Cardiomyocyte Proliferation and Heart Regeneration

Authors
Mahmoud, A.I., O'Meara, C.C., Gemberling, M., Zhao, L., Bryant, D.M., Zheng, R., Gannon, J.B., Cai, L., Choi, W.Y., Egnaczyk, G.F., Burns, C.E., Burns, C.G., MacRae, C.A., Poss, K.D., Lee, R.T.
ID
ZDB-PUB-150811-9
Date
2015
Source
Developmental Cell   34(4): 387-99 (Journal)
Registered Authors
Burns (Erter), Caroline, Burns, Geoff, Choi, Wen-Yee, Egnaczyk, Greg, Gemberling, Matt, MacRae, Calum A., Poss, Kenneth D., Zhao, Long
Keywords
none
Datasets
GEO:GSE69775
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Animals, Newborn
  • Cell Proliferation/drug effects
  • Cholinergic Neurons/physiology*
  • Denervation
  • Gene Expression Regulation/drug effects
  • Heart/innervation*
  • Heart/physiology*
  • Immunity/drug effects
  • Immunity/genetics
  • Inflammation/genetics
  • Mice
  • Models, Biological
  • Molecular Sequence Data
  • Myocytes, Cardiac/cytology*
  • Nerve Growth Factor/pharmacology
  • Neuregulin-1/pharmacology
  • Regeneration*/drug effects
  • Synaptic Transmission/drug effects
  • Vagotomy
  • Zebrafish
PubMed
26256209 Full text @ Dev. Cell
Abstract
Some organisms, such as adult zebrafish and newborn mice, have the capacity to regenerate heart tissue following injury. Unraveling the mechanisms of heart regeneration is fundamental to understanding why regeneration fails in adult humans. Numerous studies have revealed that nerves are crucial for organ regeneration, thus we aimed to determine whether nerves guide heart regeneration. Here, we show using transgenic zebrafish that inhibition of cardiac innervation leads to reduction of myocyte proliferation following injury. Specifically, pharmacological inhibition of cholinergic nerve function reduces cardiomyocyte proliferation in the injured hearts of both zebrafish and neonatal mice. Direct mechanical denervation impairs heart regeneration in neonatal mice, which was rescued by the administration of neuregulin 1 (NRG1) and nerve growth factor (NGF) recombinant proteins. Transcriptional analysis of mechanically denervated hearts revealedĀ a blunted inflammatory and immune response following injury. These findings demonstrate that nerve function is required for both zebrafish and mouse heart regeneration.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes