PUBLICATION
Collagen duplicate genes of bone and cartilage participate during regeneration of zebrafish fin skeleton
- Authors
- Duran, I., Csukasi, F., Taylor, S.P., Krakow, D., Becerra, J., Bombarely, A., Marí-Beffa, M.
- ID
- ZDB-PUB-150811-6
- Date
- 2015
- Source
- Gene expression patterns : GEP 19(1-2): 60-9 (Journal)
- Registered Authors
- Marí-Beffa, Manuel
- Keywords
- Fibrillar collagens, actinotrichia, intertrichial, lepidotrichia
- MeSH Terms
-
- Animal Fins/physiology*
- Animals
- Bone and Bones/metabolism
- Bone and Bones/physiology
- Cartilage/metabolism
- Cartilage/physiology
- Collagen/genetics
- Fibrillar Collagens/genetics*
- Fibrillar Collagens/metabolism
- Genes, Duplicate*
- Mesoderm/metabolism
- Morphogenesis/genetics
- Phylogeny
- Regeneration/genetics*
- Regeneration/physiology
- Sequence Analysis, Protein
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- PubMed
- 26256560 Full text @ Gene Expr. Patterns
Citation
Duran, I., Csukasi, F., Taylor, S.P., Krakow, D., Becerra, J., Bombarely, A., Marí-Beffa, M. (2015) Collagen duplicate genes of bone and cartilage participate during regeneration of zebrafish fin skeleton. Gene expression patterns : GEP. 19(1-2):60-9.
Abstract
The zebrafish fin is widely used as a model for skeleton regeneration. For years, the nature of the fin skeleton has been controversial as its extracellular matrix shows hybrid characteristics of both bone and cartilage. The presence of co-orthologs genes also increases the complexity of these tissues. In this article, we have identified and described the expression of fibrillar collagens in zebrafish fin skeleton. We found that genes coding for types I, II, V, XI and XXVII collagens are duplicated, showing in several cases, different expression domains. We also identified specific genomic features, such as the presence of type XXIV collagen and the absence of type III collagen in the zebrafish genome. Our study showed that actinotrichia-forming cells and osteoblasts synthesize a wide variety of these fibrillar collagens during fin regeneration. An intertrichial domain expressing most of the collagens was located in the transition between the mesenchyme condensations of actinotrichia and lepidotrichia and may determine an important niche associated with fin skeleton morphogenesis. We also confirmed the hybrid nature of the fin exoskeleton and provided a complete description of those fibrillar collagens expressed during the formation of the fin skeleton.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping