PUBLICATION

Cdon promotes neural crest migration by regulating N-cadherin localization

Authors
Powell, D.R., Williams, J.S., Hernandez-Lagunas, L., Salcedo, E., O'Brien, J.H., Bruk Artinger, K.
ID
ZDB-PUB-150811-5
Date
2015
Source
Developmental Biology   407(2): 289-99 (Journal)
Registered Authors
Powell, Davalyn
Keywords
Adhesion, Cell migration, Neural crest, Shh signaling, Zebrafish
MeSH Terms
  • Signal Transduction
  • Cadherins/metabolism*
  • Cell Surface Extensions/metabolism
  • Protein Transport
  • Torso/embryology
  • Animals
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Cell Adhesion Molecules/genetics
  • Cell Adhesion Molecules/metabolism*
  • Hedgehog Proteins/metabolism
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Cell Movement*
  • Embryo, Nonmammalian/metabolism
  • Neural Crest/cytology*
  • Neural Crest/metabolism*
  • Gene Knockdown Techniques
(all 19)
PubMed
26256768 Full text @ Dev. Biol.
Abstract
Neural crest cells (NCCs) are essential embryonic progenitor cells that are unique to vertebrates and form a remarkably complex and coordinated system of highly motile cells. Migration of NCCs occurs along specific pathways within the embryo in response to both environmental cues and cell-cell interactions within the neural crest population. Here, we demonstrate a novel role for the putative Sonic hedgehog (Shh) receptor and cell adhesion regulator, cdon, in zebrafish neural crest migration. cdon is expressed in developing premigratory NCCs but is downregulated once the cells become migratory. Knockdown of cdon results in aberrant migration of trunk NCCs: crestin positive cells can emigrate out of the neural tube but stall shortly after the initiation of migration. Live cell imaging analysis demonstrates reduced directedness of migration, increased velocity and mispositioned cell protrusions. In addition, transplantation analysis suggests that cdon is required cell-autonomously for directed NCC migration in the trunk. Interestingly, N-cadherin is mislocalized following cdon knockdown suggesting that the role of cdon in NCCs is to regulate N-cadherin localization. Our results reveal a novel role for cdon in zebrafish neural crest migration, and suggest a mechanism by which Cdon is required to localize N-cadherin to the cell membrane in migratory NCCs for directed migration.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ba2TgTransgenic Insertion
    umz30TgTransgenic Insertion
      vu234TgTransgenic Insertion
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        cdonMO6-cdonMRPHLNO
        cdonMO7-cdonMRPHLNO
        tp53MO4-tp53MRPHLNO
        1 - 3 of 3
        Show
        Fish
        Fish
        WT
        1 - 1 of 1
        Show
        Antibodies
        Name Type Antigen Genes Isotypes Host Organism
        Ab7-cdh2monoclonal
          		IgG2aMouse
          1 - 1 of 1
          Show
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          mRFPEFGmRFP
          1 - 2 of 2
          Show
          Mapping
          No data available
          Your Input Welcome
          We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
          Citation
          Powell, D.R., Williams, J.S., Hernandez-Lagunas, L., Salcedo, E., O'Brien, J.H., Bruk Artinger, K. (2015) Cdon promotes neural crest migration by regulating N-cadherin localization. Developmental Biology. 407(2):289-99.