PUBLICATION
Characterization and expression of the calpastatin gene in Cyprinus carpio
- Authors
- Chen, W.X., Ma, Y.
- ID
- ZDB-PUB-150728-1
- Date
- 2015
- Source
- Genetics and molecular research : GMR 14: 7406-7416 (Journal)
- Registered Authors
- Keywords
- Cyprinus carpio, Real-time PCR, Tissue expression, Calpastatin
- MeSH Terms
-
- Real-Time Polymerase Chain Reaction
- Conserved Sequence
- Carps/genetics*
- Carps/metabolism*
- Calcium-Binding Proteins/biosynthesis*
- Calcium-Binding Proteins/genetics*
- Molecular Sequence Data
- Cloning, Molecular
- Phylogeny
- Transcriptome
- Animals
- RNA, Messenger/genetics
- Sequence Alignment
- Open Reading Frames
- Amino Acid Sequence
- Sequence Analysis, DNA
- PubMed
- 26214419 Full text @ Genet. Mol. Res.
Citation
Chen, W.X., Ma, Y. (2015) Characterization and expression of the calpastatin gene in Cyprinus carpio. Genetics and molecular research : GMR. 14:7406-7416.
Abstract
Calpastatin, an important protein used to regulate meat quality traits in animals, is encoded by the CAST gene. The aim of the present study was to clone the cDNA sequence of the CAST gene and detect the expression of CAST in the tissues of Cyprinus carpio. The cDNA of the C. carpio CAST gene, amplified using rapid amplification of cDNA ends PCR, is 2834 bp in length (accession No. JX275386), contains a 2634-bp open reading frame, and encodes a protein with 877 amino acid residues. The amino acid sequence of the C. carpio CAST gene was 88, 80, and 59% identical to the sequences observed in grass carp, zebrafish, and other fish, respectively. The C. carpio CAST was observed to contain four conserved domains with 54 serine phosphorylation loci, 28 threonine phosphorylation loci, 1 tyrosine phosphorylation loci, and 6 specific protein kinase C phosphorylation loci. The CAST gene showed widespread expression in different tissues of C. carpio. Surprisingly, the relative expression of the CAST transcript in the muscle and heart tissues of C. carpio was significantly higher than in other tissues (P < 0.01).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping