PUBLICATION

Epoxyeicosatrienoic acids enhance embryonic haematopoiesis and adult marrow engraftment

Authors
Li, P., Lahvic, J.L., Binder, V., Pugach, E.K., Riley, E.B., Tamplin, O.J., Panigrahy, D., Bowman, T.V., Barrett, F.G., Heffner, G.C., McKinney-Freeman, S., Schlaeger, T.M., Daley, G.Q., Zeldin, D.C., Zon, L.I.
ID
ZDB-PUB-150725-11
Date
2015
Source
Nature   523: 468-71 (Journal)
Registered Authors
Bowman, Teresa, Schlaeger, Thorsten, Tamplin, Owen, Zon, Leonard I.
Keywords
Haematopoietic stem cells, Haematopoiesis, Haematological diseases
MeSH Terms
  • 8,11,14-Eicosatrienoic Acid/analogs & derivatives*
  • 8,11,14-Eicosatrienoic Acid/metabolism
  • Animals
  • Cell Line
  • Cell Movement
  • Core Binding Factor Alpha 2 Subunit/metabolism
  • Female
  • Gene Expression Regulation
  • Hematopoiesis*
  • Hematopoietic Stem Cell Transplantation*
  • Hematopoietic Stem Cells/cytology*
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Kidney/cytology
  • Male
  • Mice
  • Phosphatidylinositol 3-Kinases
  • Transcription Factor AP-1/metabolism
  • Transcription, Genetic
  • Zebrafish/embryology*
PubMed
26201599 Full text @ Nature
Abstract
Haematopoietic stem and progenitor cell (HSPC) transplant is a widely used treatment for life-threatening conditions such as leukaemia; however, the molecular mechanisms regulating HSPC engraftment of the recipient niche remain incompletely understood. Here we develop a competitive HSPC transplant method in adult zebrafish, using in vivo imaging as a non-invasive readout. We use this system to conduct a chemical screen, and identify epoxyeicosatrienoic acids (EETs) as a family of lipids that enhance HSPC engraftment. The pro-haematopoietic effects of EETs were conserved in the developing zebrafish embryo, where 11,12-EET promoted HSPC specification by activating a unique activator protein 1 (AP-1) and runx1 transcription program autonomous to the haemogenic endothelium. This effect required the activation of the phosphatidylinositol-3-OH kinase (PI(3)K) pathway, specifically PI(3)Kγ. In adult HSPCs, 11,12-EET induced transcriptional programs, including AP-1 activation, which modulate several cellular processes, such as migration, to promote engraftment. Furthermore, we demonstrate that the EET effects on enhancing HSPC homing and engraftment are conserved in mammals. Our study establishes a new method to explore the molecular mechanisms of HSPC engraftment, and discovers a previously unrecognized, evolutionarily conserved pathway regulating multiple haematopoietic generation and regeneration processes. EETs may have clinical application in marrow or cord blood transplantation.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes
This article is corrected by ZDB-PUB-220906-167 .