PUBLICATION
The dnd RNA Identifies Germ Cell Origin and Migration in Olive Flounder (Paralichthys olivaceus)
- Authors
- Wang, X., Liu, Q., Xiao, Y., Yang, Y., Wang, Y., Song, Z., You, F., An, H., Xiao, Z., Xu, S., Ma, D., Li, J.
- ID
- ZDB-PUB-150717-3
- Date
- 2015
- Source
- BioMed Research International 2015: 428591 (Journal)
- Registered Authors
- Li, Jun
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Movement/physiology*
- Flounder/metabolism*
- Gene Expression Regulation/physiology*
- Germ Cells/cytology
- Germ Cells/metabolism*
- RNA/biosynthesis*
- PubMed
- 26180800 Full text @ Biomed Res. Int.
Citation
Wang, X., Liu, Q., Xiao, Y., Yang, Y., Wang, Y., Song, Z., You, F., An, H., Xiao, Z., Xu, S., Ma, D., Li, J. (2015) The dnd RNA Identifies Germ Cell Origin and Migration in Olive Flounder (Paralichthys olivaceus). BioMed Research International. 2015:428591.
Abstract
The present study obtained a germ cell-specific marker dead end (dnd) in olive flounder (Paralichthys olivaceus) named Podnd. The tissue-specific expressions of Podnd transcripts were present in testis and ovary but were not detectable in other somatic tissues detected. SISH showed that Podnd expressed only in germ cells at different developmental stages but not in surrounding somatic cells. The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable. The Podnd transcripts localization and migration were similar to zebrafish. Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping