|ZFIN ID: ZDB-PUB-150715-9|
Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
Wehner, D., Jahn, C., Weidinger, G.
|Source:||Journal of visualized experiments : JoVE (100): e52756 (Journal)|
|Registered Authors:||Jahn, Christopher, Wehner, Daniel, Weidinger, Gilbert|
|PubMed:||26168286 Full text @ J. Vis. Exp.|
Wehner, D., Jahn, C., Weidinger, G. (2015) Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration. Journal of visualized experiments : JoVE. (100):e52756.
ABSTRACTThe zebrafish has become a very important model organism for studying vertebrate development, physiology, disease, and tissue regeneration. A thorough understanding of the molecular and cellular mechanisms involved requires experimental tools that allow for inducible, tissue-specific manipulation of gene expression or signaling pathways. Therefore, we and others have recently adapted the TetON system for use in zebrafish. The TetON system facilitates temporally and spatially-controlled gene expression and we have recently used this tool to probe for tissue-specific functions of Wnt/beta-catenin signaling during zebrafish tail fin regeneration. Here we describe the workflow for using the TetON system to achieve inducible, tissue-specific gene expression in the adult regenerating zebrafish tail fin. This includes the generation of stable transgenic TetActivator and TetResponder lines, transgene induction and techniques for verification of tissue-specific gene expression in the fin regenerate. Thus, this protocol serves as blueprint for setting up a functional TetON system in zebrafish and its subsequent use, in particular for studying fin regeneration.