PUBLICATION
Generation of Late-Born Neurons in the Ventral Spinal Cord requires the coordination of Retinoic Acid and Notch signaling
- Authors
- Ryu, J.H., Kong, H.J., Park, J.Y., Lim, K.E., An, C.M., Lee, J., Yeo, S.Y.
- ID
- ZDB-PUB-150708-2
- Date
- 2015
- Source
- Neuroscience letters 602: 95-8 (Journal)
- Registered Authors
- Yeo, Sang-Yeob
- Keywords
- Kolmer-Agduhr neuron, citral, mindbomb, retinoic acid, transgenic zebrafish
- MeSH Terms
-
- Embryo, Nonmammalian
- Animals, Genetically Modified
- Promoter Regions, Genetic
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology
- PubMed
- 26151587 Full text @ Neurosci. Lett.
Abstract
Neural progenitor cells generate various types of neurons and glia in a tightly regulated manner. During primary neurogenesis, retinoic acid (RA) acts earlier than Notch signaling and regulates differentiation and proliferation by upregulating proneural and neurogenic genes in the neural plate. However, the relationship between Notch signaling and the retinoid pathway during late neurogenesis remains unclear. We investigated the role of Mindbomb (Mib)-mediated Notch signaling in the differentiation of neural progenitors during late neurogenesis by overexpressing Mib and administering RA to Tg[hsp70-Mib:EGFP]. The majority of cells in the p3 domain differentiated into GABAergic Kolmer-Agduhr (KA) cells in Tg[hsp70-mib:EGFP]embryos heat-shocked during late neurogenesis, whereas these phenotypes were suppressed by exogenous RA. Our observations suggest that Mib-mediated Notch signaling plays a critical role in the temporal differentiation of neural progenitors, and that the generation of late-born KA" cells is regulated by the interplay between Mib and RA.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping