PUBLICATION
Bioactive C21 Steroidal Glycosides from the Roots of Cynanchum otophyllum That Suppress the Seizure-like Locomotor Activity of Zebrafish Caused by Pentylenetetrazole
- Authors
- Li, J.L., Zhou, J., Chen, Z.H., Guo, S.Y., Li, C.Q., Zhao, W.M.
- ID
- ZDB-PUB-150703-9
- Date
- 2015
- Source
- Journal of natural products 78(7): 1548-55 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Pentylenetetrazole/pharmacology*
- Cynanchum/chemistry*
- Animals
- Nuclear Magnetic Resonance, Biomolecular
- Disease Models, Animal
- Motor Activity/drug effects*
- Zebrafish
- Molecular Structure
- Pregnenes/chemistry
- Pregnenes/isolation & purification*
- Pregnenes/pharmacology*
- Plant Roots/chemistry
- Seizures/drug therapy*
- Structure-Activity Relationship
- Drugs, Chinese Herbal/chemistry
- Drugs, Chinese Herbal/isolation & purification*
- Drugs, Chinese Herbal/pharmacology*
- Glycosides/chemistry*
- Glycosides/isolation & purification*
- Glycosides/pharmacology*
- PubMed
- 26135914 Full text @ J. Nat. Prod.
Citation
Li, J.L., Zhou, J., Chen, Z.H., Guo, S.Y., Li, C.Q., Zhao, W.M. (2015) Bioactive C21 Steroidal Glycosides from the Roots of Cynanchum otophyllum That Suppress the Seizure-like Locomotor Activity of Zebrafish Caused by Pentylenetetrazole. Journal of natural products. 78(7):1548-55.
Abstract
Six new C21 steroidal glycosides, cynotophyllosides A-F (1-6), together with 16 known compounds, were isolated from the roots of Cynanchum otophyllum. The structures of the new compounds were elucidated by spectroscopic analysis and chemical methods. The three major components, otophylloside F (15), otophylloside B (17), and rostratamine 3-O-β-d-oleandropyranosyl-(1→4)-β-d-cymaropyranosyl-(1→4)-β-d-cymaropyranoside (18), suppressed the seizure-like locomotor activity caused by pentylenetetrazole in zebrafish. Preliminary structure-activity relation studies revealed that a pregnene skeleton with a C-12 ester group (ikemaoyl > cinnamoyl > hydroxy > p-hydroxybenzoyl) and a C-3 sugar chain consisting of three 2,6-dideoxysaccharide units is essential for this suppressive activity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping