PUBLICATION
Dual-slit confocal light sheet microscopy for in vivo whole-brain imaging of zebrafish
- Authors
- Yang, Z., Mei, L., Xia, F., Luo, Q., Fu, L., Gong, H.
- ID
- ZDB-PUB-150703-1
- Date
- 2015
- Source
- Biomedical Optics Express 6: 1797-1811 (Journal)
- Registered Authors
- Keywords
- (110.0110) Imaging systems, (170.2945) Illumination design, (170.3880) Medical and biological imaging, (180.1790) Confocal microscopy, (180.2520) Fluorescence microscopy
- MeSH Terms
- none
- PubMed
- 26137381 Full text @ Biomed. Opt. Express
Citation
Yang, Z., Mei, L., Xia, F., Luo, Q., Fu, L., Gong, H. (2015) Dual-slit confocal light sheet microscopy for in vivo whole-brain imaging of zebrafish. Biomedical Optics Express. 6:1797-1811.
Abstract
In vivo functional imaging at single-neuron resolution is an important approach to visualize biological processes in neuroscience. Light sheet microscopy (LSM) is a cutting edge in vivo imaging technique that provides micron-scale spatial resolution at high frame rate. Due to the scattering and absorption of tissue, however, conventional LSM is inadequate to resolve cells because of the attenuated signal to noise ratio (SNR). Using dual-beam illumination and confocal dual-slit detection, here a dual-slit confocal LSM is demonstrated to obtain the SNR enhanced images with frame rate twice as high as line confocal LSM method. Through theoretical calculations and experiments, the correlation between the slit's width and SNR was determined to optimize the image quality. In vivo whole brain structural imaging stacks and the functional imaging sequences of single slice were obtained for analysis of calcium activities at single-cell resolution. A two-fold increase in imaging speed of conventional confocal LSM makes it possible to capture the sequence of the neurons' activities and help reveal the potential functional connections in the whole zebrafish's brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping