PUBLICATION
Stereoselective Determination of Tebuconazole in Water and Zebrafish by Supercritical Fluid Chromatography tandem mass spectrometry
- Authors
- Liu, N., Dong, F., Xu, J., Liu, X., Chen, Z., Tao, Y., Pan, X., Chen, X., Zheng, Y.
- ID
- ZDB-PUB-150701-3
- Date
- 2015
- Source
- Journal of Agricultural and Food Chemistry 63(28): 6297-303 (Journal)
- Registered Authors
- Xu, Jun
- Keywords
- tebuconazole, chiral separation, supercritical fluid chromatography-tandem mass spectrometry, zebrafish
- MeSH Terms
-
- Animals
- Chromatography, Supercritical Fluid/methods*
- Fungicides, Industrial/analysis
- Fungicides, Industrial/chemistry
- Fungicides, Industrial/isolation & purification*
- Reproducibility of Results
- Sensitivity and Specificity
- Stereoisomerism
- Tandem Mass Spectrometry/methods*
- Triazoles/analysis
- Triazoles/chemistry
- Triazoles/isolation & purification*
- Water/analysis*
- Water Pollutants, Chemical/analysis
- Zebrafish*
- PubMed
- 26125486 Full text @ J. Agric. Food Chem.
Citation
Liu, N., Dong, F., Xu, J., Liu, X., Chen, Z., Tao, Y., Pan, X., Chen, X., Zheng, Y. (2015) Stereoselective Determination of Tebuconazole in Water and Zebrafish by Supercritical Fluid Chromatography tandem mass spectrometry. Journal of Agricultural and Food Chemistry. 63(28):6297-303.
Abstract
A simple and sensitive method for the enantioselective determination of tebuconazole enantiomers in water and zebrafish has been established using supercritical fluid chromatography (SFC)-MS/MS. The effects of the chiral stationary phases, mobile phase, auto back pressure regulator (ABPR) pressure, column temperature, flow rate of the mobile phase and the compensation pump solvent were evaluated. Finally, the optimal SFC-MS/MS working conditions were determined to include a CO2/MeOH mobile phase (87/13, v/v), 2.0 mL/min flow rate, 2200 psi ABPR and 30 ºC column temperature using a Chiralpak IA-3 chiral column under electrospray ionization positive mode. The modified QuEChERS method was applied to water and zebrafish samples. The mean recoveries for the tebuconazole enantiomers were 79.8% to 108.4% with RSDs ≤ 7.0% in both matrices. The LOQs ranged from 0.24 to 1.20 μg/kg. The developed analytical method was further validated by application to the analysis of authentic samples.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping