PUBLICATION
Reproductive hormones affect follicular cells and ooplasm of Stage I and II oocytes in zebrafish
- Authors
- Sousa, M.L., Silva, A., Malhão, F., Rocha, M.J., Rocha, E., Urbatzka, R.
- ID
- ZDB-PUB-150626-8
- Date
- 2016
- Source
- Reproduction, fertility, and development 28(12): 1945-1952 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cells, Cultured
- Estradiol/pharmacology*
- Estradiol/physiology
- Female
- Granulosa Cells/ultrastructure
- Hydroxyprogesterones/pharmacology*
- Oocytes/physiology*
- Ovarian Follicle/physiology*
- Testosterone/analogs & derivatives*
- Testosterone/pharmacology
- Testosterone/physiology
- Zebrafish
- PubMed
- 26108784 Full text @ Reprod. Fertil. Dev.
Citation
Sousa, M.L., Silva, A., Malhão, F., Rocha, M.J., Rocha, E., Urbatzka, R. (2016) Reproductive hormones affect follicular cells and ooplasm of Stage I and II oocytes in zebrafish. Reproduction, fertility, and development. 28(12):1945-1952.
Abstract
The basic pathway of oocyte development and its regulation is evolutionarily conserved among vertebrates; however, little is known about the role of hormones at the first stages (Stages I and II) of follicle development in fish. In the present study, zebrafish follicles at Stages I and II were exposed in vitro to the reproductive hormones 17β-oestradiol (E2), 11-ketotestosterone (11KT), 17,20β-dihydroxy-4-pregnen-3-one (DHP) and to the secondary messenger dibutyryl cyclic adenosine monophosphate (db-cAMP) at a concentration of 1µM for a 48-h period. Morphological alterations of the ooplasm were assessed by transmission electron microscopy and of the granulosa cell layer by quantitative stereology. Expression of mRNA was analysed for cell-cycle genes (cyclin B and E) and resident proteins of the endoplasmic reticulum (calnexin and 78-kDa glucose-regulated protein (grp78/bip)). E2 and db-cAMP stimulated the presence of endoplasmic reticulum in the ooplasm and calnexin mRNA increased in the db-cAMP treatment, but also in response to 11KT and DHP. 11KT, DHP and db-cAMP inhibited the progression of the cell cycle in the granulosa-theca cell layer, indicated by a reduction of the nucleus volume-weighted size of granulosa cells and of increased cyclin E mRNA expression. Reproductive hormones had different effects on the ooplasm and the granulosa-theca cell layer of zebrafish follicles, predominantly at Stage II.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping