PUBLICATION
A naturally monomeric infrared fluorescent protein for protein labeling in vivo
- Authors
- Yu, D., Baird, M.A., Allen, J.R., Howe, E.S., Klassen, M.P., Reade, A., Makhijani, K., Song, Y., Liu, S., Murthy, Z., Zhang, S.Q., Weiner, O.D., Kornberg, T.B., Jan, Y.N., Davidson, M.W., Shu, X.
- ID
- ZDB-PUB-150623-3
- Date
- 2015
- Source
- Nature Methods 12(8): 763-5 (Journal)
- Registered Authors
- Reade, Anna
- Keywords
- Fluorescence imaging, Fluorescent proteins, Cellular imaging, Transgenic organisms
- MeSH Terms
-
- Mutation
- Plasmids/metabolism
- Fluorescent Dyes/chemistry
- Histidine/chemistry
- Transfection
- Infrared Rays*
- Neurons/metabolism
- Green Fluorescent Proteins/chemistry*
- Animals
- Developmental Biology
- Zebrafish
- Molecular Sequence Data
- DNA/chemistry
- Amino Acid Sequence
- Drosophila melanogaster
- Luminescent Proteins/chemistry
- Humans
- Animals, Genetically Modified
- Recombinant Fusion Proteins/chemistry
- Protein Multimerization
- HeLa Cells
- Protein Conformation
- Mice
- PubMed
- 26098020 Full text @ Nat. Methods
Citation
Yu, D., Baird, M.A., Allen, J.R., Howe, E.S., Klassen, M.P., Reade, A., Makhijani, K., Song, Y., Liu, S., Murthy, Z., Zhang, S.Q., Weiner, O.D., Kornberg, T.B., Jan, Y.N., Davidson, M.W., Shu, X. (2015) A naturally monomeric infrared fluorescent protein for protein labeling in vivo. Nature Methods. 12(8):763-5.
Abstract
Infrared fluorescent proteins (IFPs) provide an additional color to GFP and its homologs in protein labeling. Drawing on structural analysis of the dimer interface, we identified a bacteriophytochrome in the sequence database that is monomeric in truncated form and engineered it into a naturally monomeric IFP (mIFP). We demonstrate that mIFP correctly labels proteins in live cells, Drosophila and zebrafish. It should be useful in molecular, cell and developmental biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping