PUBLICATION
A naturally monomeric infrared fluorescent protein for protein labeling in vivo
- Authors
- Yu, D., Baird, M.A., Allen, J.R., Howe, E.S., Klassen, M.P., Reade, A., Makhijani, K., Song, Y., Liu, S., Murthy, Z., Zhang, S.Q., Weiner, O.D., Kornberg, T.B., Jan, Y.N., Davidson, M.W., Shu, X.
- ID
- ZDB-PUB-150623-3
- Date
- 2015
- Source
- Nature Methods 12(8): 763-5 (Journal)
- Registered Authors
- Reade, Anna
- Keywords
- Fluorescence imaging, Fluorescent proteins, Cellular imaging, Transgenic organisms
- MeSH Terms
-
- Recombinant Fusion Proteins/chemistry
- HeLa Cells
- Molecular Sequence Data
- Animals, Genetically Modified
- Green Fluorescent Proteins/chemistry*
- Histidine/chemistry
- Amino Acid Sequence
- Fluorescent Dyes/chemistry
- Drosophila melanogaster
- Infrared Rays*
- Developmental Biology
- Protein Conformation
- Transfection
- Protein Multimerization
- Neurons/metabolism
- Humans
- Mice
- Zebrafish
- Animals
- DNA/chemistry
- Mutation
- Luminescent Proteins/chemistry
- Plasmids/metabolism
- PubMed
- 26098020 Full text @ Nat. Methods
Citation
Yu, D., Baird, M.A., Allen, J.R., Howe, E.S., Klassen, M.P., Reade, A., Makhijani, K., Song, Y., Liu, S., Murthy, Z., Zhang, S.Q., Weiner, O.D., Kornberg, T.B., Jan, Y.N., Davidson, M.W., Shu, X. (2015) A naturally monomeric infrared fluorescent protein for protein labeling in vivo. Nature Methods. 12(8):763-5.
Abstract
Infrared fluorescent proteins (IFPs) provide an additional color to GFP and its homologs in protein labeling. Drawing on structural analysis of the dimer interface, we identified a bacteriophytochrome in the sequence database that is monomeric in truncated form and engineered it into a naturally monomeric IFP (mIFP). We demonstrate that mIFP correctly labels proteins in live cells, Drosophila and zebrafish. It should be useful in molecular, cell and developmental biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping