PUBLICATION

The chromatin remodeler chd5 is necessary for proper head development during embryogenesis of Danio rerio

Authors
Bishop, B., Ho, K.K., Tyler, K., Smith, A., Bonilla, S., Leung, Y.F., Ogas, J.
ID
ZDB-PUB-150621-4
Date
2015
Source
Biochimica et biophysica acta. Gene regulatory mechanisms   1849(8): 1040-50 (Journal)
Registered Authors
Bonilla, Sylvia, Leung, Yuk Fai, Ogas, Joe, Smith, Amanda
Keywords
ATP-dependent chromatin remodeling, CHD remodeler, embryogenesis, neural development
MeSH Terms
  • Adenosine Triphosphatases/genetics
  • Animals
  • Animals, Genetically Modified
  • Brain/embryology
  • Brain/metabolism
  • Cartilage/embryology
  • Cartilage/metabolism
  • Cell Differentiation/genetics
  • Chromatin Assembly and Disassembly/genetics
  • DNA Helicases/genetics
  • DNA Helicases/physiology*
  • Dopaminergic Neurons/physiology
  • Embryo, Nonmammalian
  • Embryonic Development/genetics*
  • Eye/embryology
  • Eye/metabolism
  • Head/embryology*
  • Neurogenesis/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed
26092436 Full text @ BBA Gene Regulatory Mechanisms
Abstract
The chromatin remodeler CHD5 plays a critical role in tumor suppression and neurogenesis in mammals. CHD5 contributes to gene expression during neurogenesis, but there is still much to learn regarding how this class of remodelers contributes to differentiation and development. CHD5 remodelers are vertebrate-specific, raising the prospect that CHD5 plays one or more conserved roles in this phylum. Expression of chd5 in adult fish closely mirrors expression of CHD5 in adult mammals. Knockdown of Chd5 during embryogenesis suggests new roles for CHD5 remodelers based on resulting defects in craniofacial development including reduced head and eye size as well as reduced cartilage formation in the head. In addition, knockdown of Chd5 results in altered expression of neural markers in the developing brain and eye as well as a profound defect in differentiation of dopaminergic amacrine cells. Recombinant zebrafish Chd5 protein exhibits nucleosome remodeling activity in vitro, suggesting that it is the loss of this activity that contributes to the observed phenotypes. Our studies indicate that zebrafish is an appropriate model for functional characterization of CHD5 remodelers in vertebrates and highlight the potential of this model for generating novel insights into the role of this vital class of remodelers.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping