PUBLICATION

Unpredictable Chronic Stress Alters Adenosine Metabolism in Zebrafish Brain

Authors
Zimmermann, F.F., Altenhofen, S., Kist, L.W., Leite, C.E., Bogo, M.R., Cognato, G.P., Bonan, C.D.
ID
ZDB-PUB-150618-5
Date
2016
Source
Molecular neurobiology   53(4): 2518-28 (Journal)
Registered Authors
Bonan, Carla Denise
Keywords
Adenosine, Adenosine deaminase, Ectonucleotidases, Unpredictable chronic stress, Zebrafish
MeSH Terms
  • Adenosine/metabolism*
  • Adenosine Deaminase/genetics
  • Adenosine Deaminase/metabolism
  • Adenosine Triphosphatases/metabolism
  • Adenosine Triphosphate/metabolism
  • Animals
  • Brain/metabolism*
  • Brain/pathology*
  • Chronic Disease
  • Enzyme Assays
  • Extracellular Space/metabolism
  • Gene Expression Regulation
  • Hydrolysis
  • Male
  • Stress, Psychological/metabolism*
  • Zebrafish/metabolism*
PubMed
26081145 Full text @ Mol. Neurobiol.
Abstract
Stress is considered a risk factor for several human disorders. Despite the broad knowledge of stress responses in mammals, data on the relationship between unpredictable chronic stress (UCS) and its effects on purinergic signaling are limited. ATP hydrolysis by ectonucleotidases is an important source of adenosine, and adenosine deaminase (ADA) contributes to the control of the nucleoside concentrations. Considering that some stress models could affect signaling systems, the objective of this study was to investigate whether UCS alters ectonucleotidase and ADA pathway in zebrafish brain. Additionally, we analyzed ATP metabolism as well as ada1, ada2.1, ada2.2, adaL, and adaasi gene expression in zebrafish brain. Our results have demonstrated that UCS did not alter ectonucleotidase and soluble ADA activities. However, ecto-ADA activity was significantly decreased (26.8 %) in brain membranes of animals exposed to UCS when compared to the control group. Quantitative reverse transcription PCR (RT-PCR) analysis did not show significant changes on ADA gene expression after the UCS exposure. The brain ATP metabolism showed a marked increase in adenosine levels (ADO) in animals exposed to UCS. These data suggest an increase on extracellular adenosine levels in zebrafish brain. Since this nucleoside has neuromodulatory and anxiolytic effects, changes in adenosine levels could play a role in counteracting the stress, which could be related to a compensatory mechanism in order to restore the homeostasis.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping