Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-PUB-150614-1
Defining the phenotype of neutrophils following reverse migration in zebrafish
Ellett, F., Elks, P.M., Robertson, A.L., Ogryzko, N.V., Renshaw, S.A.
Date: 2015
Source: Journal of Leukocyte Biology   98(6): 975-81 (Journal)
Registered Authors: Elks, Phil, Ellett, Felix, Ogryzko, Nikolay, Renshaw, Steve A., Robertson, Anne
Keywords: chemotaxis, granulocytes, infection, inflammation, phagocytosis
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/immunology
  • Cell Movement/genetics
  • Cell Movement/immunology*
  • Inflammation/genetics
  • Inflammation/immunology
  • Neutrophils/immunology*
  • Staphylococcus aureus/immunology
  • Zebrafish/genetics
  • Zebrafish/immunology*
PubMed: 26071567 Full text @ J. Leukoc. Biol.
Stimulation of neutrophil reverse migration presents an attractive, alternative therapeutic pathway to driving inflammation resolution. However, little is known about whether the activity of wound-experienced neutrophils is altered and whether encouraging dispersal of such neutrophils back into the body may have undesirable consequences. This study used a zebrafish tail transection inflammation model, in combination with a photoconvertible neutrophil transgenic line, to allow internally controlled, simultaneous comparison of reverse-migrated neutrophils with naïve neutrophils in the presence and absence of secondary insult. Detailed microscopy revealed that reverse-migrated neutrophils exhibited an activated morphology but responded normally to secondary insult and are able to mount an effective antimicrobial response to Staphylococcus aureus. These results support a model in which reverse-migrated neutrophils exhibit no long-term behavioral alterations and encourage the notion of enhanced reverse migration as a viable target for pharmaceutical manipulation.