|ZFIN ID: ZDB-PUB-150614-1|
Defining the phenotype of neutrophils following reverse migration in zebrafish
Ellett, F., Elks, P.M., Robertson, A.L., Ogryzko, N.V., Renshaw, S.A.
|Source:||Journal of Leukocyte Biology 98(6): 975-81 (Journal)|
|Registered Authors:||Elks, Phil, Ellett, Felix, Ogryzko, Nikolay, Renshaw, Steve A., Robertson, Anne|
|Keywords:||chemotaxis, granulocytes, infection, inflammation, phagocytosis|
|PubMed:||26071567 Full text @ J. Leukoc. Biol.|
Ellett, F., Elks, P.M., Robertson, A.L., Ogryzko, N.V., Renshaw, S.A. (2015) Defining the phenotype of neutrophils following reverse migration in zebrafish. Journal of Leukocyte Biology. 98(6):975-81.
ABSTRACTStimulation of neutrophil reverse migration presents an attractive, alternative therapeutic pathway to driving inflammation resolution. However, little is known about whether the activity of wound-experienced neutrophils is altered and whether encouraging dispersal of such neutrophils back into the body may have undesirable consequences. This study used a zebrafish tail transection inflammation model, in combination with a photoconvertible neutrophil transgenic line, to allow internally controlled, simultaneous comparison of reverse-migrated neutrophils with naïve neutrophils in the presence and absence of secondary insult. Detailed microscopy revealed that reverse-migrated neutrophils exhibited an activated morphology but responded normally to secondary insult and are able to mount an effective antimicrobial response to Staphylococcus aureus. These results support a model in which reverse-migrated neutrophils exhibit no long-term behavioral alterations and encourage the notion of enhanced reverse migration as a viable target for pharmaceutical manipulation.