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ZFIN ID: ZDB-PUB-150610-3
2-bromopalmitate impairs neural stem/progenitor cell proliferation, promotes cell apoptosis and induces malformation in zebrafish embryonic brain
Wang, C., Chen, X., Shi, W., Wang, F., Du, Z., Li, X., Yao, Y., Liu, T., Shao, T., Li, G., Hao, A.
Date: 2015
Source: Neurotoxicology and teratology 50: 53-63 (Journal)
Registered Authors:
Keywords: 2-bromopalmitate, FGF signaling pathway, neural development, neural stem/progenitor cells, palmitoylation, zebrafish
MeSH Terms:
  • Animals
  • Apoptosis/drug effects*
  • Brain/drug effects*
  • Brain/pathology
  • Cell Proliferation/drug effects*
  • Embryo, Nonmammalian/drug effects
  • MAP Kinase Signaling System/drug effects
  • Neural Stem Cells/drug effects*
  • Palmitates/toxicity*
  • Zebrafish/embryology
  • Zebrafish/genetics
PubMed: 26056731 Full text @ Neurotoxicol. Teratol.
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ABSTRACT
2-bromopalmitate (2BP) is a widely used palmitoylation inhibitor. Besides, it has been reported that 2BP can inhibit T-cell activation, making it a potential immunosuppressor for treatment of autoimmune diseases. Although the important roles of palmitoylation in neural system have been noted during past decades, the effect of 2BP on neural development is still not very clear. In this study, we demonstrated that 25uM-100uM 2BP exposure caused apparent neural malformation in the presumptive brains of zebrafish embryos at 14 hpf. Further studies implied that the mRNA quantities and distributions of neural stem/progenitor cell (NSPC) markers (neurog1, sox2, sox3) in the affected regions of 50uM 2BP treated embryos significantly decreased. In addition, we found that 2BP impaired the NSPC proliferation at 10 hpf and 14 hpf as well as promoted cell apoptosis at 14 hpf, consistent with which the interference with FGF/ERK signaling pathway was also detected. For the first time, this study provided information about the toxicity and teratogenicity of 2BP for neural development in vivo.
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