ZFIN ID: ZDB-PUB-150527-3
Sesamol decreases melanin biosynthesis in melanocyte cells and zebrafish: Possible involvement of MITF via the intracellular cAMP and p38/JNK signalling pathways
Baek, S.H., Lee, S.H.
Date: 2015
Source: Experimental dermatology 24(10): 761-6 (Journal)
Registered Authors:
Keywords: antimelanogenic agents, cyclic adenosine monophosphate, microphthalmia-associated transcription factor, sesamol, tyrosinase, zebrafish
MeSH Terms:
  • Animals
  • Antioxidants/pharmacology*
  • Benzodioxoles/pharmacology*
  • Cyclic AMP/metabolism
  • Dose-Response Relationship, Drug
  • Down-Regulation
  • Gene Expression/drug effects*
  • Intramolecular Oxidoreductases/genetics
  • JNK Mitogen-Activated Protein Kinases/metabolism
  • Melanins/biosynthesis*
  • Melanocytes/drug effects*
  • Melanocytes/metabolism
  • Microphthalmia-Associated Transcription Factor/genetics
  • Monophenol Monooxygenase/genetics
  • Monophenol Monooxygenase/metabolism
  • Oxidoreductases/genetics
  • Phenols/pharmacology*
  • Phosphorylation/drug effects
  • Pigmentation/drug effects
  • Protein Biosynthesis/drug effects*
  • Receptor, Melanocortin, Type 1/genetics
  • Zebrafish
  • p38 Mitogen-Activated Protein Kinases/metabolism
PubMed: 26010596 Full text @ Exp. Dermatol.
ABSTRACT
The development of anti-melanogenic agents is important for the prevention of serious esthetic problems such as melasma, freckles, age spots, and chloasma. The aim of this study was to investigate the anti-melanogenic effect of sesamol, an active lignan isolated from Sesamum indicum, in melan-a cells. Sesamol strongly inhibited melanin biosynthesis and the activity of intracellular tyrosinase by decreasing cyclic adenosine monophosphate (cAMP) accumulation. Sesamol significantly decreased the expression of melanogenesis-related genes, such as tyrosinase, tyrosinase-related protein-1,2 (TRP-1,2), microphthalmia-associated transcription factor (MITF), and melanocortin 1 receptor (MC1R). In addition, sesamol also induces phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) and c-Jun N-terminal kinase (JNK). Moreover, sesamol dose-dependently decreased zebrafish pigment formation, tyrosinase activity, and expression of melanogenesis-related genes. These findings indicate that sesamol inhibited melanin biosynthesis by down-regulating tyrosinase activity and melanin production via regulation of gene expression of melanogenesis-related proteins through modulation of MITF activity, which promoted phosphorylation of p38 and JNK in melan-a cells. Together, these results suggest that sesamol strongly inhibits melanin biosynthesis, and therefore sesamol represents a new skin-whitening agent for use in cosmetics. This article is protected by copyright. All rights reserved.
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