PUBLICATION
Second generation physical and linkage maps of yellowtail (Seriola quinqueradiata) and comparison of synteny with four model fish
- Authors
- Aoki, J.Y., Kai, W., Kawabata, Y., Ozaki, A., Yoshida, K., Koyama, T., Sakamoto, T., Araki, K.
- ID
- ZDB-PUB-150525-2
- Date
- 2015
- Source
- BMC Genomics 16: 406 (Journal)
- Registered Authors
- Araki, Kazuo
- Keywords
- none
- MeSH Terms
-
- Animals
- Evolution, Molecular
- Gene Expression Profiling
- Genetic Linkage
- Genome
- Models, Animal
- Oryzias/genetics*
- Perciformes/genetics*
- Phylogeny
- Polymorphism, Single Nucleotide
- Radiation Hybrid Mapping/methods*
- Synteny*
- Tetraodontiformes/genetics*
- Zebrafish/genetics*
- PubMed
- 26003112 Full text @ BMC Genomics
Citation
Aoki, J.Y., Kai, W., Kawabata, Y., Ozaki, A., Yoshida, K., Koyama, T., Sakamoto, T., Araki, K. (2015) Second generation physical and linkage maps of yellowtail (Seriola quinqueradiata) and comparison of synteny with four model fish. BMC Genomics. 16:406.
Abstract
Background Physical and linkage maps are important aids for the assembly of genome sequences, comparative analyses of synteny, and to search for candidate genes by quantitative trait locus analysis. Yellowtail, Seriola quinqueradiata, is an economically important species in Japanese aquaculture, and genetic information will be useful for DNA-assisted breeding. We report the construction of a second generation radiation hybrid map, its synteny analysis, and a second generation linkage map containing SNPs (single nucleotide polymorphisms) in yellowtail.
Results Approximately 1.4 million reads were obtained from transcriptome sequence analysis derived from 11 tissues of one individual. To identify SNPs, cDNA libraries were generated from a pool of 500 whole juveniles, and the gills and kidneys of 100 adults. 9,356 putative SNPs were detected in 6,025 contigs, with a minor allele frequency ≥25%. The linkage and radiation hybrid maps were constructed based on these contig sequences. 2,081 markers, including 601 SNPs markers, were mapped onto the linkage map, and 1,532 markers were mapped in the radiation hybrid map.
Conclusions The second generation linkage and physical maps were constructed using 6,025 contigs having SNP markers. These maps will aid the de novo assembly of sequencing reads, linkage studies and the identification of candidate genes related to important traits. The comparison of marker contigs in the radiation hybrid map indicated that yellowtail is evolutionarily closer to medaka than to green-spotted pufferfish, three-spined stickleback or zebrafish. The synteny analysis may aid studies of chromosomal evolution in yellowtail compared with model fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping