PUBLICATION

Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation

Authors

Li, W.H., Zhou, L., Li, Z., Wang, Y., Shi, J.T., Yang, Y.J., Gui, J.F.

ID

ZDB-PUB-150523-5

Date

2015

Source

International journal of biological sciences 11(6): 688-700 (Journal)

Registered Authors

Li, Zhi, Wang, Yang, Zhou, Li

Keywords

otx2, Lbh-like, Notch, photoreceptor differentiation, retina development

MeSH Terms

Molecular Sequence Data
Photoreceptor Cells, Vertebrate/cytology*
Apoptosis
Trans-Activators/genetics
Trans-Activators/metabolism
Trans-Activators/physiology*
Sequence Alignment
Amino Acid Sequence
Eye/anatomy & histology
Eye/embryology
Otx Transcription Factors/genetics
Otx Transcription Factors/metabolism
Otx Transcription Factors/physiology*
Gene Knockdown Techniques
Animals
Cell Proliferation
Cell Differentiation/genetics*
Zebrafish/genetics
Zebrafish/growth & development*
Genetic Markers
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism
Zebrafish Proteins/physiology*

(all 23)

PubMed

25999792 Full text @ Int. J. Biol. Sci.

Abstract

The homeobox transcription factor orthodenticle homolog 2 (otx2) is supposed as an organizer that orchestrates a transcription factor network during photoreceptor development. However, its regulation in the process remains unclear. In this study, we have identified a zebrafish limb bud and heart-like gene (lbh-like), which is expressed initially at 30 hours post fertilization (hpf) in the developing brain and eyes. Lbh-like knockdown by morpholinos specifically inhibits expression of multiple photoreceptor-specific genes, such as opsins, gnat1, gnat2 and irbp. Interestingly, otx2 expression in the morphants is not significantly reduced until 32 hpf when lbh-like begins to express, but its expression level in 72 hpf morphants is higher than that in wild type embryos. Co-injection of otx2 and its downstream target neuroD mRNAs can rescue the faults in eyes of Lbh-like morphants. Combined with the results of promoter-reporter assay, we suggest that lbh-like is a new regulator of photoreceptor differentiation directly through affecting otx2 expression in zebrafish. Furthermore, knockdown of lbh-like increases the activity of Notch pathway and perturbs the balance among proliferation, differentiation and survival of photoreceptor precursors.

Genes / Markers

Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO1-lbhl	standard conditions	Prim-15	brain otx2b expression decreased amount, abnormal	Fig. 7
AB + MO1-lbhl	standard conditions	Prim-15	retina otx2b expression decreased amount, abnormal	Fig. 7
AB + MO1-lbhl	standard conditions	Prim-25	brain otx2b expression decreased amount, abnormal	Fig. 7
AB + MO1-lbhl	standard conditions	Prim-25	retina otx2b expression decreased amount, abnormal	Fig. 7
AB + MO1-lbhl	standard conditions	Long-pec	brain her15.1 expression increased amount, abnormal	Fig. 6
AB + MO1-lbhl	standard conditions	Long-pec	brain her15.2 expression increased amount, abnormal	Fig. 6
AB + MO1-lbhl	standard conditions	Long-pec	brain notch1a expression increased amount, abnormal	Fig. 6
AB + MO1-lbhl	standard conditions	Long-pec	brain otx2b expression decreased amount, abnormal	Fig. 7
AB + MO1-lbhl	standard conditions	Long-pec	ciliary marginal zone cell population proliferation increased process quality, abnormal	Fig. 6
AB + MO1-lbhl	standard conditions	Long-pec	eye decreased diameter, abnormal	Fig. 2

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Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
lbhl	MO1-lbhl	MRPHLNO
otx2b	MO1-otx2b	MRPHLNO
tp53	MO4-tp53	MRPHLNO

Fish

Fish
AB
AB + MO1-lbhl
AB + MO1-lbhl + MO1-otx2b
WT

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
AB1-lbhl	polyclonal	lbhl		Rabbit

Orthology

Engineered Foreign Genes

Mapping

Li et al., 2015 ZDB-PUB-150523-5 PMID:25999792

Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation

Li et al., 2015

ZDB-PUB-150523-5
PMID:25999792