PUBLICATION
Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation
- Authors
- Li, W.H., Zhou, L., Li, Z., Wang, Y., Shi, J.T., Yang, Y.J., Gui, J.F.
- ID
- ZDB-PUB-150523-5
- Date
- 2015
- Source
- International journal of biological sciences 11(6): 688-700 (Journal)
- Registered Authors
- Li, Zhi, Wang, Yang, Zhou, Li
- Keywords
- otx2, Lbh-like, Notch, photoreceptor differentiation, retina development
- MeSH Terms
-
- Apoptosis
- Gene Knockdown Techniques
- Animals
- Sequence Alignment
- Molecular Sequence Data
- Amino Acid Sequence
- Photoreceptor Cells, Vertebrate/cytology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- Trans-Activators/genetics
- Trans-Activators/metabolism
- Trans-Activators/physiology*
- Otx Transcription Factors/genetics
- Otx Transcription Factors/metabolism
- Otx Transcription Factors/physiology*
- Genetic Markers
- Cell Differentiation/genetics*
- Cell Proliferation
- Eye/anatomy & histology
- Eye/embryology
- Zebrafish/genetics
- Zebrafish/growth & development*
- PubMed
- 25999792 Full text @ Int. J. Biol. Sci.
Citation
Li, W.H., Zhou, L., Li, Z., Wang, Y., Shi, J.T., Yang, Y.J., Gui, J.F. (2015) Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation. International journal of biological sciences. 11(6):688-700.
Abstract
The homeobox transcription factor orthodenticle homolog 2 (otx2) is supposed as an organizer that orchestrates a transcription factor network during photoreceptor development. However, its regulation in the process remains unclear. In this study, we have identified a zebrafish limb bud and heart-like gene (lbh-like), which is expressed initially at 30 hours post fertilization (hpf) in the developing brain and eyes. Lbh-like knockdown by morpholinos specifically inhibits expression of multiple photoreceptor-specific genes, such as opsins, gnat1, gnat2 and irbp. Interestingly, otx2 expression in the morphants is not significantly reduced until 32 hpf when lbh-like begins to express, but its expression level in 72 hpf morphants is higher than that in wild type embryos. Co-injection of otx2 and its downstream target neuroD mRNAs can rescue the faults in eyes of Lbh-like morphants. Combined with the results of promoter-reporter assay, we suggest that lbh-like is a new regulator of photoreceptor differentiation directly through affecting otx2 expression in zebrafish. Furthermore, knockdown of lbh-like increases the activity of Notch pathway and perturbs the balance among proliferation, differentiation and survival of photoreceptor precursors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping