PUBLICATION

Molecular Cloning and Functional Characterization of Mannose Receptor in Zebra Fish (Danio rerio) during Infection with Aeromonas sobria

Authors

Zheng, F., Asim, M., Lan, J., Zhao, L., Wei, S., Chen, N., Liu, X., Zhou, Y., Lin, L.

ID

ZDB-PUB-150520-4

Date

2015

Source

International Journal of Molecular Sciences 16: 10997-11012 (Journal)

Registered Authors

Keywords

Aeromonas sobria, Danio rerio, mRNA expression, mannose receptor, protein expression

MeSH Terms

Molecular Sequence Data
Aeromonas hydrophila/physiology*
Animals
Immunohistochemistry
RNA, Messenger/metabolism
Spleen/metabolism
Interleukin-1beta/metabolism
Receptors, Cell Surface/classification
Receptors, Cell Surface/genetics
Receptors, Cell Surface/metabolism*
Zebrafish/metabolism*
Zebrafish/microbiology
Mannose-Binding Lectins/classification
Mannose-Binding Lectins/genetics
Mannose-Binding Lectins/metabolism*
Gram-Negative Bacterial Infections/metabolism
Gram-Negative Bacterial Infections/pathology
Gram-Negative Bacterial Infections/veterinary
Cloning, Molecular
Phylogeny
Sequence Alignment
Real-Time Polymerase Chain Reaction
Intestines/metabolism
Liver/metabolism
Amino Acid Sequence
Humans
Kidney/metabolism
Tumor Necrosis Factor-alpha/metabolism
Lectins, C-Type/classification
Lectins, C-Type/genetics
Lectins, C-Type/metabolism*

(all 31)

PubMed

25988382 Full text @ Int. J. Mol. Sci.

Abstract

Mannose receptor (MR) is a member of pattern-recognition receptors (PRRs), which plays a significant role in immunity responses. Much work on MR has been done in mammals and birds while little in fish. In this report, a MR gene (designated as zfMR) was cloned from zebra fish (Danio rerio), which is an attractive model for the studies of animal diseases. The full-length cDNA of zfMR contains 6248 bp encoding a putative protein of 1428 amino acids. The predicted amino acid sequences showed that zfMR contained a cysteine-rich domain, a single fibronectin type II (FN II) domain, eight C-type lectin-like domains (CTLDs), a transmembrane domain and a short C-terminal cytoplasmic domain, sharing highly conserved structures with MRs from the other species. The MR mRNA could be detected in all examined tissues with highest level in kidney. The temporal expression patterns of MR, IL-1β and TNF-α mRNAs were analyzed in the liver, spleen, kidney and intestine post of infection with Aeromonas sobria. By immunohistochemistry assay, slight enhancement of MR protein was also observed in the spleen and intestine of the infected zebra fish. The established zebra fish-A. sobria infection model will be valuable for elucidating the role of MR in fish immune responses to infection.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT	bacterial treatment by injection: Aeromonas	Adult	intestine Ab1-mrc1 labeling increased amount, abnormal	Fig. 5
WT	bacterial treatment by injection: Aeromonas	Adult	kidney il1b expression increased amount, abnormal	Fig. 4
WT	bacterial treatment by injection: Aeromonas	Adult	kidney mrc1b expression increased amount, abnormal	Fig. 4
WT	bacterial treatment by injection: Aeromonas	Adult	kidney tnfa expression increased amount, abnormal	Fig. 4
WT	bacterial treatment by injection: Aeromonas	Adult	liver il1b expression increased amount, abnormal	Fig. 4
WT	bacterial treatment by injection: Aeromonas	Adult	liver mrc1b expression increased amount, abnormal	Fig. 4
WT	bacterial treatment by injection: Aeromonas	Adult	liver tnfa expression increased amount, abnormal	Fig. 4
WT	bacterial treatment by injection: Aeromonas	Adult	spleen il1b expression increased amount, abnormal	Fig. 4
WT	bacterial treatment by injection: Aeromonas	Adult	spleen mrc1b expression increased amount, abnormal	Fig. 4
WT	bacterial treatment by injection: Aeromonas	Adult	spleen tnfa expression increased amount, abnormal	Fig. 4

1 - 10 of 11

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Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
WT

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-mrc1				Rabbit

Orthology

Gene	Orthology
mrc1b

Engineered Foreign Genes

Mapping

Zheng et al., 2015 ZDB-PUB-150520-4 PMID:25988382

Molecular Cloning and Functional Characterization of Mannose Receptor in Zebra Fish (Danio rerio) during Infection with Aeromonas sobria

Zheng et al., 2015

ZDB-PUB-150520-4
PMID:25988382