PUBLICATION
Assessment of immunotoxicity of dibutyl phthalate using live zebrafish embryos
- Authors
- Xu, H., Dong, X., Zhang, Z., Yang, M., Wu, X., Liu, H., Lao, Q., Li, C.
- ID
- ZDB-PUB-150510-5
- Date
- 2015
- Source
- Fish & shellfish immunology 45(2): 286-92 (Journal)
- Registered Authors
- Keywords
- Dibutyl phthalate, Immune cells, Phthalate esters, zebrafish
- MeSH Terms
-
- Animals
- Dibutyl Phthalate/toxicity*
- Embryo, Nonmammalian/immunology
- Random Allocation
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/immunology*
- PubMed
- 25956719 Full text @ Fish Shellfish Immunol.
Citation
Xu, H., Dong, X., Zhang, Z., Yang, M., Wu, X., Liu, H., Lao, Q., Li, C. (2015) Assessment of immunotoxicity of dibutyl phthalate using live zebrafish embryos. Fish & shellfish immunology. 45(2):286-92.
Abstract
This study set out to understand the immune-toxic effects of dibutyl phthalate (DBP) using transgenic, albino or AB line zebrafish. Zebrafish embryos were exposed to different concentrations of DBP, and the immune cells formation, phagocytosis ability were measured after a short-term exposure to DBP for 6 h post-fertilization (hpf) to 72 or 96 hpf. Exposure to DBP was found to inhibit the neutrophils and macrophage formation in a concentration-dependent manner. The ability of macrophage phagocytosis was all decreased after exposure to DBP, indicating the occurrence of immunotoxicity. The respiratory burst was induced, and the transcription levels of T/B cell-related genes rag1/2 were up-regulated. The overall results indicate that DBP in aquatic environment greatly influence the immune system in fish, and zebrafish embryos can serve as a reliable model for the developmental immunotoxicity of toxic-chemicals.
Errata / Notes
Correction.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping