PUBLICATION
Hydrogen sulfide promotes calcium uptake in larval zebrafish
- Authors
- Kwong, R.W., Perry, S.F.
- ID
- ZDB-PUB-150508-6
- Date
- 2015
- Source
- American journal of physiology. Cell physiology 309(1): C60-9 (Journal)
- Registered Authors
- Perry, Steve F.
- Keywords
- Calcium, ECaC, hydrogen sulfide, protein kinase, zebrafish
- MeSH Terms
-
- Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors
- Cyclic AMP-Dependent Protein Kinases/metabolism
- Organothiophosphorus Compounds/pharmacology*
- Time Factors
- Oligonucleotides, Antisense/metabolism
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Animals
- Morpholinos/metabolism
- Larva/drug effects
- Larva/metabolism
- Animals, Genetically Modified
- Calcium/metabolism*
- Gene Knockdown Techniques
- Hydrogen Sulfide/pharmacology*
- Cystathionine beta-Synthase/genetics
- Cystathionine beta-Synthase/metabolism
- Protein Kinase Inhibitors/pharmacology
- Morpholines/pharmacology*
- Ion Transport
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Sulfides/pharmacology*
- Sodium-Potassium-Exchanging ATPase/metabolism
- PubMed
- 25948733 Full text @ Am. J. Physiol. Cell Physiol.
Citation
Kwong, R.W., Perry, S.F. (2015) Hydrogen sulfide promotes calcium uptake in larval zebrafish. American journal of physiology. Cell physiology. 309(1):C60-9.
Abstract
Hydrogen sulfide (H2S) can act as a signalling molecule for various ion channels and/or transporters, however, little is known about its potential involvement in Ca(2+) balance. Using developing zebrafish (Danio rerio) as an in vivo model system, the present study demonstrated that acute exposure to H2S donors increased Ca(2+) influx at 4 days post fertilization (dpf) while chronic (3-day) exposure caused a rise in whole-body Ca(2+) levels. The mRNA expression of Ca(2+)-transport related genes was unaffected by H2S exposure, suggesting that post-transcriptional modifications were responsible for the altered rates of Ca(2+) uptake. Indeed, treatment of fish with the protein kinase A (PKA) inhibitor H-89 abolished the H2S-mediated stimulation of Ca(2+) influx, suggesting that H2S increased Ca(2+) influx by activating cAMP-PKA pathways. Cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) are two key enzymes in the endogenous synthesis of H2S. Using an antisense morpholino knockdown approach, we demonstrated that Ca(2+) influx was reduced in CBSb- but not in CSE-deficient fish. Interestingly, the reduction in Ca(2+) influx in CBSb-deficient fish was observed only in fish that were acclimated to low Ca(2+) water (i.e., 25 µM Ca(2+); control: 250 µM Ca(2+)). Similarly, mRNA expression of cbsb but not cse was increased in fish acclimated to low Ca(2+) water. Results from whole-mount immunohistochemistry further revealed that CBSb was expressed in Na(+)/K(+)-ATPase-rich cells (NaR cells), which are implicated in Ca(2+) uptake in zebrafish larvae. Collectively, the present study suggests a novel role for H2S in promoting Ca(2+) influx, particularly in a low Ca(2+) environment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping