PUBLICATION
Mesoscopic in vivo 3-D tracking of sparse cell populations using angular multiplexed optical projection tomography
- Authors
- Chen, L., Alexandrov, Y., Kumar, S., Andrews, N., Dallman, M.J., French, P.M., McGinty, J.
- ID
- ZDB-PUB-150425-4
- Date
- 2015
- Source
- Biomedical Optics Express 6: 1253-61 (Journal)
- Registered Authors
- Keywords
- (170.2520) Fluorescence microscopy, (170.3010) Image reconstruction techniques, (170.6900) Three-dimensional microscopy, (170.6920) Time-resolved imaging
- MeSH Terms
- none
- PubMed
- 25909009 Full text @ Biomed. Opt. Express
Citation
Chen, L., Alexandrov, Y., Kumar, S., Andrews, N., Dallman, M.J., French, P.M., McGinty, J. (2015) Mesoscopic in vivo 3-D tracking of sparse cell populations using angular multiplexed optical projection tomography. Biomedical Optics Express. 6:1253-61.
Abstract
We describe an angular multiplexed imaging technique for 3-D in vivo cell tracking of sparse cell distributions and optical projection tomography (OPT) with superior time-lapse resolution and a significantly reduced light dose compared to volumetric time-lapse techniques. We demonstrate that using dual axis OPT, where two images are acquired simultaneously at different projection angles, can enable localization and tracking of features in 3-D with a time resolution equal to the camera frame rate. This is achieved with a 200x reduction in light dose compared to an equivalent volumetric time-lapse single camera OPT acquisition with 200 projection angles. We demonstrate the application of this technique to mapping the 3-D neutrophil migration pattern observed over ~25.5 minutes in a live 2 day post-fertilisation transgenic LysC:GFP zebrafish embryo following a tail wound.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping