PUBLICATION
A mitochondrial targeted two-photon iridium(III) phosphorescent probe for selective detection of hypochlorite in live cells and in vivo
- Authors
- Li, G., Lin, Q., Sun, L., Feng, C., Zhang, P., Yu, B., Chen, Y., Wen, Y., Wang, H., Ji, L., Chao, H.
- ID
- ZDB-PUB-150422-12
- Date
- 2015
- Source
- Biomaterials 53: 285-95 (Journal)
- Registered Authors
- Chen, Yu, Wang, Hui
- Keywords
- Hypochlorite, Iridium(III) complex, Mitochondria, Two-photon probe, Zebrafish
- MeSH Terms
-
- Animals
- Cell Line
- Humans
- Hypochlorous Acid/analysis*
- Iridium/pharmacology*
- Limit of Detection
- Luminescence
- Mice
- Mitochondria/drug effects*
- Molecular Probes
- Photons
- Zebrafish
- PubMed
- 25890727 Full text @ Biomaterials
Citation
Li, G., Lin, Q., Sun, L., Feng, C., Zhang, P., Yu, B., Chen, Y., Wen, Y., Wang, H., Ji, L., Chao, H. (2015) A mitochondrial targeted two-photon iridium(III) phosphorescent probe for selective detection of hypochlorite in live cells and in vivo. Biomaterials. 53:285-95.
Abstract
Endogenous hypochlorite ion (ClO(-)) is a highly reactive oxygen species (ROS) that is produced from hydrogen peroxide and chloride ions catalyzed by myeloperoxidase (MPO). And mitochondrion is one of the major sources of ROS including ClO(-). In the present work, a two-photon phosphorescent probe for ClO(-) in mitochondria was developed. An iridium(III) complex bearing a diaminomaleonitrile group as ClO(-) reactive moiety specifically responded to ClO(-) over other ions and ROSs. When the probe was reacted with ClO(-) to form an oxidized carboxylate product, a significant enhancement in phosphorescence intensity was observed under one-photon (402 nm) and two-photon (750 nm) excitation, with a two-photon absorption cross-section of 78.1 GM at 750 nm. More importantly, ICP-MS results and cellular images co-stained with Mito-tracker Green demonstrated that this probe possessed high specificity for mitochondria. This probe was applied in the one- and two-photon imaging of ClO(-) in vitro and in vivo. The results suggested endotoxin lipopolysaccharide (LPS) induced ClO(-) mostly generated in the liver of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping