PUBLICATION

Parental trophic exposure to three aromatic fractions of polycyclic aromatic hydrocarbons in the zebrafish: Consequences for the offspring

Authors
Perrichon, P., Akcha, F., Le Menach, K., Goubeau, M., Budzinski, H., Cousin, X., Bustamante, P.
ID
ZDB-PUB-150419-1
Date
2015
Source
The Science of the total environment   524-525C: 52-62 (Journal)
Registered Authors
Cousin, Xavier
Keywords
Cardiac performance, Fish early-life stages, Genotoxicity, PAH metabolites, Parental transmission, PhotoMotor Response, Polycyclic aromatic hydrocarbons
MeSH Terms
  • Animals
  • Biomarkers/metabolism
  • Biotransformation
  • DNA Damage
  • Environmental Monitoring*
  • Estuaries
  • Food Chain*
  • Larva
  • Polycyclic Aromatic Hydrocarbons/analysis*
  • Polycyclic Aromatic Hydrocarbons/metabolism
  • Water Pollutants, Chemical/analysis*
  • Water Pollutants, Chemical/metabolism
  • Zebrafish/metabolism*
PubMed
25889544 Full text @ Sci. Total Environ.
Abstract
In recent decades, PAH emissions due to extensive anthropogenic activities have risen sharply causing considerable pollution of aquatic ecosystems. This pollution represents a threat for organisms, among them are fish. Consequently, prenatal stress can have important repercussions, and may impact survival and population recruitment. To investigate this point, eggs were collected from zebrafish exposed during 6months by trophic route to three aromatic fractions from two different origins, pyrolytic (PY) and petrogenic (light (BAL) and heavy (HFO) fractions) sources. Chronic dietary exposure of the parents was performed at environmentally relevant concentrations (0.3×, 1× and 3×; 1× represents an environmental concentration measured in French estuary). In order to explore the consequences of parental exposure for the next first generation, toxic responses were studied in both embryos and larvae using a multiscale approach. Toxic effects were assessed by looking at hatching success, developmental abnormalities, photomotor response and heartbeat. The level of PAH metabolites and EROD activity in fish larvae were measured to assess exposure to PAHs. Egg production of parents was significantly reduced compared to the Control; hence little information was available for BAL and HFO offspring. The size of larvae from PY parents was found to increase despite a reduced yolk sac compared to Control larvae. Furthermore, a high level of behavioral stress was observed in larvae originating from parents exposed to three-fold the environmental concentration. The cardiac activity was reduced in a concentration-dependent manner for the PY exposure group. No effect was however observed on biotransformation markers (cyp1a, EROD), nor on the level of DNA damage for all PY, BAL and HFO offspring. The absence of significant differences in metabolite levels may indicate a potential early depuration of transferred compounds or no PAH-transmission. The disruptions observed at the individual level in the next generation could impact on the longer-term, surviving population.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping