PUBLICATION
Contributions from extracellular sources of adenosine to the ethanol toxicity in zebrafish larvae
- Authors
- Lutte, A.H., Capiotti, K.M., Silva, N.L., Silva, C.S., Kist, L.W., Bogo, M.R., Silva, R.S.
- ID
- ZDB-PUB-150418-8
- Date
- 2015
- Source
- Reproductive toxicology (Elmsford, N.Y.) 53: 82-91 (Journal)
- Registered Authors
- Keywords
- Adenosine, Zebrafish., ecto-5′-nucleotidase, Ethanol
- MeSH Terms
-
- 5'-Nucleotidase/antagonists & inhibitors
- 5'-Nucleotidase/genetics
- 5'-Nucleotidase/metabolism*
- Adenosine Diphosphate/analogs & derivatives*
- Adenosine Diphosphate/pharmacology
- Animals
- Dipyridamole/pharmacology
- Ethanol/toxicity*
- Larva/anatomy & histology
- Larva/drug effects*
- Larva/physiology
- Motor Activity/drug effects
- Zebrafish/abnormalities
- Zebrafish/physiology
- PubMed
- 25883026 Full text @ Reprod. Toxicol.
- CTD
- 25883026
Citation
Lutte, A.H., Capiotti, K.M., Silva, N.L., Silva, C.S., Kist, L.W., Bogo, M.R., Silva, R.S. (2015) Contributions from extracellular sources of adenosine to the ethanol toxicity in zebrafish larvae. Reproductive toxicology (Elmsford, N.Y.). 53:82-91.
Abstract
The effects of ethanol exposure on extracellular adenosine sources in zebrafish were evaluated. In the acute treatment, the embryos were exposed to 2% ethanol on day 1 post-fertilization (dpf). In the chronic treatment, the exposure was continued for 2h/day up to 6dpf. Ecto-5'-nucleotidase activity was assessed by colorimetric method and gene expression determined by RT-qPCR in 7dpf zebrafish. Body length, ocular distance and surface area of the eyes were registered in animals acutely exposed to ethanol and pretreated with AOPCP (5 to 500nM), an ecto-5'-nucleotidase inhibitor, or dipyridamole (10 to 100μM), a blocker of nucleoside transport. Both ethanol exposures promoted increased ecto-5'-nucleotidase activity, impaired locomotion and morphology. Ecto-5'-nucleotidase expression was not affected. AOPCP promoted mild prevention of morphological defects caused by acute treatment, while dipyridamole worsened these defects. Early ethanol exposure altered adenosinergic tonus, especially through nucleoside transporters, contributing to morphological defects produced by ethanol in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping