PUBLICATION

Response to Nodal morphogen gradient is determined by the kinetics of target gene induction

Authors
Dubrulle, J., Jordan, B.M., Akhmetova, L., Farrell, J.A., Kim, S.H., Solnica-Krezel, L., Schier, A.F.
ID
ZDB-PUB-150415-7
Date
2015
Source
eLIFE   4: (Journal)
Registered Authors
Dubrulle, Julien, Farrell, Jeffrey, Kim, Seok-Hyung, Schier, Alexander, Solnica-Krezel, Lilianna
Keywords
DNA binding affinity, developmental biology, morphogen gradient, signal transduction, stem cells, transcription rate, zebrafish
Datasets
GEO:GSE67648
MeSH Terms
  • Animals
  • Body Patterning/drug effects
  • Body Patterning/genetics
  • Endoderm/drug effects
  • Endoderm/metabolism
  • Female
  • Gene Expression Regulation, Developmental/drug effects
  • Green Fluorescent Proteins/metabolism
  • Kinetics
  • Mice
  • Models, Biological
  • Nodal Protein/pharmacology*
  • Signal Transduction/drug effects
  • Signal Transduction/genetics
  • Smad2 Protein/metabolism
  • Time Factors
  • Transcription, Genetic/drug effects
  • Transcriptional Activation*
  • Transgenes
  • Zebrafish/embryology
  • Zebrafish/genetics
PubMed
25869585 Full text @ Elife
Abstract
Morphogen gradients expose cells to different signal concentrations and induce target genes with different ranges of expression. To determine how the Nodal morphogen gradient induces distinct gene expression patterns during zebrafish embryogenesis, we measured the activation dynamics of the signal transducer Smad2 and the expression kinetics of long- and short-range target genes. We found that threshold models based on ligand concentration are insufficient to predict the response of target genes. Instead, morphogen interpretation is shaped by the kinetics of target gene induction: the higher the rate of transcription and the earlier the onset of induction, the greater the spatial range of expression. Thus, the timing and magnitude of target gene expression can be used to modulate the range of expression and diversify the response to morphogen gradients.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes