PUBLICATION
Robust regeneration of adult zebrafish lateral line hair cells reflects continued precursor pool maintenance
- Authors
- Cruz, I.A., Kappedal, R., Mackenzie, S.M., Hailey, D.W., Hoffman, T.L., Schilling, T.F., Raible, D.W.
- ID
- ZDB-PUB-150415-6
- Date
- 2015
- Source
- Developmental Biology 402(2): 229-38 (Journal)
- Registered Authors
- Raible, David, Schilling, Tom
- Keywords
- Adult zebrafish, hair cells, lateral line, neuromasts, regeneration
- MeSH Terms
-
- Animals
- Bromodeoxyuridine
- Fluorescence
- Immunohistochemistry
- Lateral Line System/cytology*
- Lateral Line System/physiology*
- Mechanoreceptors/physiology*
- Neomycin
- Regeneration/physiology*
- Zebrafish/physiology*
- PubMed
- 25869855 Full text @ Dev. Biol.
Citation
Cruz, I.A., Kappedal, R., Mackenzie, S.M., Hailey, D.W., Hoffman, T.L., Schilling, T.F., Raible, D.W. (2015) Robust regeneration of adult zebrafish lateral line hair cells reflects continued precursor pool maintenance. Developmental Biology. 402(2):229-38.
Abstract
We have examined lateral line hair cell and support cell maintenance in adult zebrafish when growth is largely complete. We demonstrate that adult zebrafish not only replenish hair cells after a single instance of hair cell damage, but also maintain hair cells and support cells after multiple rounds of damage and regeneration. We find that hair cells undergo continuous turnover in adult zebrafish in the absence of damage. We identify mitotically-distinct support cell populations and show that hair cells regenerate from underlying support cells in a region-specific manner. Our results demonstrate that there are two distinct support cell populations in the lateral line, which may help explain why zebrafish hair cell regeneration is extremely robust, retained throughout life, and potentially unlimited in regenerative capacity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping