PUBLICATION
Benzo(a)pyrene-induced cytochrome p4501A expression of four freshwater fishes (Oryzias latipes, Danio rerio, Cyprinus carpio, and Zacco platypus)
- Authors
- Lee, J.W., Yoon, H.G., Lee, S.K.
- ID
- ZDB-PUB-150412-2
- Date
- 2015
- Source
- Environmental Toxicology and Pharmacology 39: 1041-1050 (Journal)
- Registered Authors
- Keywords
- Biomarker, Cyprinus carpio, Cytochrome P450 1A, Danio rerio, Oryzias latipes, Zacco platypus
- MeSH Terms
-
- Dose-Response Relationship, Drug
- Phylogeny
- Environmental Pollutants/adverse effects
- Environmental Pollutants/pharmacology
- Cytochrome P-450 CYP1A1/genetics*
- Cytochrome P-450 CYP1A1/metabolism*
- Liver/enzymology
- Benzo(a)pyrene/adverse effects*
- Benzo(a)pyrene/pharmacology
- Carps/genetics
- Carps/growth & development
- Carps/metabolism
- Gene Expression Regulation, Enzymologic/drug effects
- Cyprinidae/classification
- Cyprinidae/genetics
- Cyprinidae/growth & development*
- Cyprinidae/metabolism
- Fish Proteins/genetics
- Fish Proteins/metabolism
- Animals
- Gills/enzymology
- Oryzias/genetics
- Oryzias/growth & development*
- Oryzias/metabolism
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/metabolism
- PubMed
- 25863331 Full text @ Environ. Toxicol. Pharmacol.
Citation
Lee, J.W., Yoon, H.G., Lee, S.K. (2015) Benzo(a)pyrene-induced cytochrome p4501A expression of four freshwater fishes (Oryzias latipes, Danio rerio, Cyprinus carpio, and Zacco platypus). Environmental Toxicology and Pharmacology. 39:1041-1050.
Abstract
Oryzias latipes, Danio rerio, Cyprinus carpio, and Zacco platypus are useful indicator species for CYP1A biomarker studies; however, comparative studies have not been performed. To compare susceptibility, dose- and time-dependent CYP1A induction at the mRNA and protein levels in response to benzo(a)pyrene (BaP) exposure was analyzed. At the mRNA level, a statistically significant difference was found among the four species; however, such was not observed at the protein level. C. carpio showed the highest CYP1A induction level and the steepest slope in the dose-response curve. To assess susceptibility, the difference in CYP1A mRNA induction among species must be considered, and C. carpio was the most sensitive species of the four evaluated in terms of CYP1A expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping