PUBLICATION
TBBPA induces developmental toxicity, oxidative stress, and apoptosis in embryos and zebrafish larvae (Danio rerio)
- Authors
- Wu, S., Ji, G., Liu, J., Zhang, S., Gong, Y., Shi, L.
- ID
- ZDB-PUB-150408-13
- Date
- 2016
- Source
- Environmental toxicology 31(10): 1241-9 (Journal)
- Registered Authors
- Keywords
- TBBPA, apoptosis, developmental toxicity, oxidative stress, zebrafish
- MeSH Terms
-
- Glutathione Peroxidase/metabolism
- Apoptosis/drug effects*
- Heart/drug effects
- Animals
- Larva/drug effects
- Larva/metabolism
- Polybrominated Biphenyls/toxicity*
- Superoxide Dismutase/metabolism
- Myocardium/pathology
- Flame Retardants/toxicity*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Zebrafish/genetics
- Zebrafish/growth & development
- Brain/drug effects
- Brain/pathology
- Catalase/metabolism
- Down-Regulation/drug effects
- Oxidative Stress/drug effects*
- PubMed
- 25846749 Full text @ Env. Tox.
- CTD
- 25846749
Citation
Wu, S., Ji, G., Liu, J., Zhang, S., Gong, Y., Shi, L. (2016) TBBPA induces developmental toxicity, oxidative stress, and apoptosis in embryos and zebrafish larvae (Danio rerio). Environmental toxicology. 31(10):1241-9.
Abstract
Tetrabromobisphenol A (TBBPA) is currently one of the most frequently used brominated flame retardants and can be considered as a high production volume chemical. In this study, zebrafish embryos and larvae served as a biological model to evaluate TBBPA-induced developmental toxicity, oxidative stress, oxidant-associated gene expression, and cell apoptosis. Abnormalities, including hyperemia and pericardial edema, were induced in zebrafish larvae. The results showed that toxicity endpoints such as hatching rate, survival rate, malformation rate, and growth rate had a significant dose-response relationship with TBBPA. Further studies revealed that TBBPA did not alter the enzyme activities of Copper/Zinc Superoxide dismutase (Cu/Zn-SOD), catalase (CAT), and glutathioneperoxidase (GPx) at 0.10 mg/L, but decreased activities following exposure to 0.40, 0.70, and 1.00 mg/L. Despite the significantly decreased gene expression of Cu/Zn-SOD, CAT, and GPx1a in the 1.00 mg/L treatment group, other treatments (0.10, 0.40, 0.70 mg/L) did not alter gene expression. Moreover, Acridine orange staining results showed that apoptotic cells mainly accumulated in the brain, heart, and tail, indicating possible TBBPA-induced brain, cardiac, and blood circulation system impairment in zebrafish embryos and larvae. Histological analysis also showed evidence of obvious heart impairment in TBBPA-treated groups. This study provides new evidence on the developmental toxicity, oxidative stress, and apoptosis of embryos and zebrafish larvae, which is important for the evaluation of environmental toxicity and chemical risk.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping