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ZFIN ID: ZDB-PUB-150326-8
Effects of postprandial starvation on mRNA expression of endocrine-, amino acid and peptide transporter-, and metabolic enzyme-related genes in zebrafish (Danio rerio)
Tian, J., He, G., Mai, K., Liu, C.
Date: 2015
Source: Fish physiology and biochemistry 41(3): 773-87 (Journal)
Registered Authors:
Keywords: none
MeSH Terms:
  • Alanine Transaminase/metabolism
  • Amino Acid Transport Systems/genetics
  • Amino Acid Transport Systems/metabolism
  • Analysis of Variance
  • Animals
  • Aspartate Aminotransferases/metabolism
  • Cholecystokinin/metabolism
  • DNA Primers/genetics
  • Fasting/metabolism*
  • Fatty Acid Synthases/metabolism
  • Feedback, Physiological/physiology
  • Gene Expression Regulation/physiology*
  • Glutamate Dehydrogenase/metabolism
  • Glutamate-Ammonia Ligase/metabolism
  • Leptin/metabolism
  • Lipoprotein Lipase/metabolism
  • Membrane Transport Proteins/genetics
  • Membrane Transport Proteins/metabolism
  • Neuropeptide Y/metabolism
  • Postprandial Period/physiology*
  • RNA, Messenger/metabolism*
  • Real-Time Polymerase Chain Reaction
  • Time Factors
  • Trypsin/metabolism
  • Zebrafish/metabolism
  • Zebrafish/physiology*
PubMed: 25805459 Full text @ Fish Physiol. Biochem.
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ABSTRACT
The goal of this study was to systematically evaluate the molecular activities of endocrine-, amino acid and peptide transporters-, and metabolic enzyme-related genes in 35-day-old mixed-sex zebrafish (Danio rerio) after feeding . Zebrafish with initial body weights ranging from 9 to 11 mg were fasted for 384 h in a controlled indoor environment. Fish were sampled at 0, 3, 6, 12, 24, 48, 96, 192, and 384 h after fed. Overall, the present study results show that the regulatory mechanism that insulin-like growth factor I negative feedback regulated growth hormone is conserved in zebrafish, as it is in mammals, but that regulation of growth hormone receptors is highly intricate. Leptin and cholecystokinin are time-dependent negative feedback signals, and neuropeptide Y may be an important positive neuropeptide for food intake in zebrafish. The amino acid/carnitine transporters B(0,+) (ATB(0,+)) and broad neutral (0) amino acid transporter 1(B(0)AT1) mRNA levels measured in our study suggest that protein may be utilized during 24-96 h of fasting in zebrafish. Glutamine synthetase mRNA levels were downregulated, and glutamate dehydrogenase, alanine aminotransferase, aspartate transaminase, and trypsin mRNA levels were upregulated after longtime fasting in this study. The mRNA expression levels of fatty acid synthetase decreased significantly (P < 0.05), whereas those of lipoprotein lipase rapidly increased after 96 h of fasting. Fasting activated the expression of glucose synthesis genes when fasting for short periods of time; when fasting is prolonged, the mRNA levels of glucose breakdown enzymes and pentose phosphate shunt genes decreased.
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