ZFIN ID: ZDB-PUB-150325-1
Red light-regulated reversible nuclear localization of proteins in mammalian cells and zebrafish
Beyer, H.M., Juillot, S., Herbst, K., Samodelov, S.L., Müller, K., Schamel, W.W., Römer, W., Schäfer, E., Nagy, F., Straehle, U., Weber, W., Zurbriggen, M.D.
Date: 2015
Source: ACS synthetic biology   4(9): 951-8 (Journal)
Registered Authors: Strähle, Uwe
Keywords: light-inducible nuclear transport, optogenetics, phytochrome, mammalian, synthetic biology, plant synthetic biology
MeSH Terms:
  • Active Transport, Cell Nucleus/radiation effects*
  • Animals
  • Arabidopsis/metabolism
  • Arabidopsis Proteins/metabolism
  • CHO Cells
  • Cell Nucleus/radiation effects*
  • Cricetulus
  • Genetic Engineering*
  • Light*
  • Mice
  • NIH 3T3 Cells
  • Phytochrome B/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Zebrafish
PubMed: 25803699 Full text @ ACS Synth Biol
Protein trafficking in and out of the nucleus represents a key step in controlling cell fate and function. Here we report the development of a red light-inducible and far-red light-reversible synthetic system for controlling nuclear localization of proteins in mammalian cells and zebrafish. First, we synthetically reconstructed and validated the red light-dependent Arabidopsis phytochrome B nuclear import mediated by phytochrome-interacting factor 3 in a non-plant environment and support current hypotheses on the import mechanism in planta. Based on this principle we next regulated nuclear import and activity of target proteins by the spatiotemporal projection of light patterns. A synthetic transcription factor was translocated into the nucleus of mammalian cells and zebrafish to drive transgene expression. These data demonstrate the first in vivo application of a plant phytochrome-based optogenetic tool in vertebrates and expand the repertoire of available light-regulated molecular devices.