ZFIN ID: ZDB-PUB-150318-5
PAPC mediates self/non-self-distinction during Snail1-dependent tissue separation
Luu, O., Damm, E.W., Parent, S.E., Barua, D., Smith, T.H., Wen, J.W., Lepage, S.E., Nagel, M., Ibrahim-Gawel, H., Huang, Y., Bruce, A.E., Winklbauer, R.
Date: 2015
Source: The Journal of cell biology   208: 839-856 (Other)
Registered Authors: Bruce, Ashley, Damm, Erich W., Lepage, Stephanie, Smith, Tamara
Keywords: none
MeSH Terms:
  • Actins/metabolism
  • Animals
  • Body Patterning
  • Cadherins/physiology*
  • Cell Adhesion
  • Cell Polarity
  • Gastrula/embryology
  • Gastrula/metabolism
  • Mesoderm/cytology
  • Mesoderm/metabolism
  • Receptors, G-Protein-Coupled/metabolism
  • Transcription Factors/physiology*
  • Xenopus Proteins/metabolism
  • Xenopus Proteins/physiology*
  • Xenopus laevis
  • Zebrafish
  • Zebrafish Proteins/physiology
PubMed: 25778923 Full text @ J. Cell Biol.
Cleft-like boundaries represent a type of cell sorting boundary characterized by the presence of a physical gap between tissues. We studied the cleft-like ectoderm-mesoderm boundary in Xenopus laevis and zebrafish gastrulae. We identified the transcription factor Snail1 as being essential for tissue separation, showed that its expression in the mesoderm depends on noncanonical Wnt signaling, and demonstrated that it enables paraxial protocadherin (PAPC) to promote tissue separation through two novel functions. First, PAPC attenuates planar cell polarity signaling at the ectoderm-mesoderm boundary to lower cell adhesion and facilitate cleft formation. Second, PAPC controls formation of a distinct type of adhesive contact between mesoderm and ectoderm cells that shows properties of a cleft-like boundary at the single-cell level. It consists of short stretches of adherens junction-like contacts inserted between intermediate-sized contacts and large intercellular gaps. These roles of PAPC constitute a self/non-self-recognition mechanism that determines the site of boundary formation at the interface between PAPC-expressing and -nonexpressing cells.