PUBLICATION
Diastereoisomer-specific effects of hexabromocyclododecanes on hepatic aryl hydrocarbon receptors and cytochrome P450s in zebrafish (Danio rerio)
- Authors
- Du, M., Fang, C., Qiu, L., Dong, S., Zhang, X., Yan, C.
- ID
- ZDB-PUB-150317-10
- Date
- 2015
- Source
- Chemosphere 132: 24-31 (Journal)
- Registered Authors
- Keywords
- Aryl hydrocarbon receptor, Cytochrome P450, Diastereoisomer, Hexabromocyclododecane
- MeSH Terms
-
- Animals
- Cytochrome P-450 CYP1A1/metabolism
- Cytochrome P-450 Enzyme System/metabolism*
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Cytochrome P-450 CYP1B1
- Receptors, Aryl Hydrocarbon/metabolism*
- Liver/metabolism
- Hydrocarbons, Brominated/adverse effects*
- PubMed
- 25770833 Full text @ Chemosphere
Citation
Du, M., Fang, C., Qiu, L., Dong, S., Zhang, X., Yan, C. (2015) Diastereoisomer-specific effects of hexabromocyclododecanes on hepatic aryl hydrocarbon receptors and cytochrome P450s in zebrafish (Danio rerio). Chemosphere. 132:24-31.
Abstract
In order to elucidate the mechanism for diastereoisomer-specific toxicity and metabolism of hexabromocyclododecanes (HBCDs) in biota, zebrafish (Danio rerio) were exposed to different concentrations of individual HBCD diastereoisomers (α-, β- and γ-HBCD) in water for 7 and 21d. We examined the gene expression of aryl hydrocarbon receptor (AHR) and cytochrome P450 (CYP), as well as ethoxyresorufin-O-deethylase (EROD) activity in zebrafish livers. Exposure to different HBCD diastereoisomers caused different expression of AHRs in zebrafish livers. For instance, 10 and 100μgL(-1) of α- and β-HBCD up-regulated the expressions of ahr1a and ahr1b in zebrafish liver, whereas 10 and 100μgL(-)(1) of γ-HBCD down-regulated them after 7d exposure. α-HBCD showed the most significant up-regulation of ahr1a and ahr1b expression, whereas γ-HBCD showed the most significant down-regulation of their expression among three HBCD diastereoisomers. Moreover, HBCDs could affect the expression of CYP1s as well as EROD activity in a gene-specific and diastereoisomer-specific manner. α-, β- and γ-HBCD inhibited cyp1a expression but enhanced the expression of cyp1b1 and cyp1c1. α-, β- and γ-HBCD showed different degrees of effect on the same CYP1 gene in a concentration-dependent way. The different effects of HBCD diastereoisomers on these genes we examined and EROD activity not only indicate diastereoisomer-specific toxic effect, but also in turn explain diastereoisomer-specific accumulation of HBCDs in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping