PUBLICATION

Mortality Caused by Bath Exposure of Zebrafish (Danio rerio) Larvae to Nervous Necrosis Virus Is Limited to the Fourth Day Postfertilization

Authors
Morick, D., Faigenbaum, O., Smirnov, M., Fellig, Y., Inbal, A., Kotler, M.
ID
ZDB-PUB-150311-18
Date
2015
Source
Applied and environmental microbiology   81(10): 3280-7 (Journal)
Registered Authors
Inbal, Adi
Keywords
none
MeSH Terms
  • Animals
  • Female
  • Fertilization
  • Fish Diseases/mortality*
  • Fish Diseases/physiopathology
  • Fish Diseases/virology
  • Larva/growth & development*
  • Larva/virology
  • Male
  • Molecular Sequence Data
  • Nodaviridae/physiology*
  • RNA Virus Infections/mortality
  • RNA Virus Infections/physiopathology
  • RNA Virus Infections/veterinary*
  • RNA Virus Infections/virology
  • Zebrafish/growth & development
  • Zebrafish/physiology
  • Zebrafish/virology*
PubMed
25746990 Full text @ Appl. Environ. Microbiol.
Abstract
Nervous necrosis virus (NNV) is a member of the Betanodavirus family that causes fatal diseases in over 40 species of fish worldwide. Mortality among NNV-infected fish larvae is almost 100%. In order to elucidate the mechanisms responsible for the susceptibility of fish larvae to NNV, we exposed zebrafish larvae to NNV by bath-immersion at 2, 4, 6 and 8 days post-fertilization (dpf). Here, we demonstrate that developing zebrafish embryos are resistant to NNV at 2 dpf due to protection afforded by the egg chorion and, to a lesser extent, by the perivitellin fluid. The zebrafish larvae succumbed to NNV infection during a narrow time "window" around the 4(th) dpf, while 6- and 8-day-old larvae were much less sensitive, with mortalities of 24% and 28%, respectively.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes