PUBLICATION
Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy
- Authors
- Lisse, T.S., Brochu, E.A., Rieger, S.
- ID
- ZDB-PUB-150306-5
- Date
- 2015
- Source
- Journal of visualized experiments : JoVE (95): (Journal)
- Registered Authors
- Rieger, Sandra
- Keywords
- none
- MeSH Terms
-
- Tail/physiology
- Microscopy/methods
- Zebrafish/physiology*
- Regeneration/physiology*
- Animals
- PubMed
- 25742070 Full text @ J. Vis. Exp.
Citation
Lisse, T.S., Brochu, E.A., Rieger, S. (2015) Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy. Journal of visualized experiments : JoVE. (95).
Abstract
The zebrafish larval tail fin is ideal for studying tissue regeneration due to the simple architecture of the larval fin-fold, which comprises of two layers of skin that enclose undifferentiated mesenchyme, and because the larval tail fin regenerates rapidly within 2-3 days. Using this system, we demonstrate a method for capturing the repair dynamics of the amputated tail fin with time-lapse video brightfield stereomicroscopy. We demonstrate that fin amputation triggers a contraction of the amputation wound and extrusion of cells around the wound margin, leading to their subsequent clearance. Fin regeneration proceeds from proximal to distal direction after a short delay. In addition, developmental growth of the larva can be observed during all stages. The presented method provides an opportunity for observing and analyzing whole tissue-scale behaviors such as fin development and growth in a simple microscope setting, which is easily adaptable to any stereomicroscope with time-lapse capabilities.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping