PUBLICATION

Brain area-specific diurnal and photic regulation of val-opsinA and val-opsinB genes in the zebrafish

Authors
Hang, C.Y., Kitahashi, T., Parhar, I.S.
ID
ZDB-PUB-150303-9
Date
2015
Source
Journal of neurochemistry   133(4): 501-510 (Journal)
Registered Authors
Keywords
light-sensitive, non-image-forming, non-visual photoreceptor, rhythmic
MeSH Terms
  • Gene Expression Regulation/drug effects
  • Gene Expression Regulation/radiation effects*
  • Male
  • Brain/anatomy & histology
  • Brain/metabolism*
  • Eye/metabolism
  • RNA, Messenger/metabolism
  • Tryptamines/pharmacology
  • Zebrafish
  • Animals
  • Circadian Rhythm/physiology*
  • Photic Stimulation*
  • Receptors, Melatonin/antagonists & inhibitors
  • Receptors, Melatonin/metabolism
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Molecular Sequence Data
  • Opsins/genetics
  • Opsins/metabolism*
(all 19)
PubMed
25727787 Full text @ J. Neurochem.
Abstract
Zebrafish possess two isoforms of vertebrate ancient long (VAL)-opsin, val-opsinA (valopa) and val-opsinB (valopb), which probably mediate non-visual responses to light. To understand the diurnal and light-sensitive regulation of the valop genes in different cell groups, the current study used real-time quantitative PCR to examine the diurnal changes of valopa and b mRNA levels in different brain areas of adult male zebrafish. Furthermore, the effects of extended exposure to light or dark condition, luminous levels, and treatment with a melatonin receptor agonist or antagonist on valop transcription were examined. In the thalamus, valop mRNA levels showed significant diurnal changes; valopa peaked in the evening, while valopb peaked in the morning. Diurnal changes in valopa mRNA levels occurred independent of light conditions, whereas those in valopb mRNA levels were regulated by light. The melatonin receptor agonist or antagonist did not affect changes in valop mRNA levels. In contrast, the midbrain and hindbrain showed arrhythmic valop mRNA levels under light and dark cycles. The differential diurnal regulation of the valopa and b genes in the thalamus and the arrhythmic expression in the midbrain and hindbrain suggest involvement of deep brain VAL-opsin in time- and light-dependent physiology. This article is protected by copyright. All rights reserved.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Hang, C.Y., Kitahashi, T., Parhar, I.S. (2015) Brain area-specific diurnal and photic regulation of val-opsinA and val-opsinB genes in the zebrafish. Journal of neurochemistry. 133(4):501-510.