PUBLICATION
Toxicity induced by Basic Violet 14, Direct Red 28 and Acid Red 26 in zebrafish larvae
- Authors
- Shen, B., Liu, H.C., Ou, W.B., Eilers, G., Zhou, S.M., Meng, F.G., Li, C.Q., Li, Y.Q.
- ID
- ZDB-PUB-150303-8
- Date
- 2015
- Source
- Journal of applied toxicology : JAT 35(12): 1473-80 (Journal)
- Registered Authors
- Keywords
- Acid Red 26, Basic Violet 14, Direct Red 28, toxicity, zebrafish
- MeSH Terms
-
- Animal Use Alternatives
- Animals
- Azo Compounds/toxicity*
- Congo Red/toxicity*
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/drug effects*
- Gene Expression Regulation, Developmental/drug effects
- Heart/drug effects
- Heart/embryology
- Larva
- Lethal Dose 50
- Liver/drug effects
- Liver/embryology
- Liver/ultrastructure
- Rosaniline Dyes/toxicity*
- Toxicity Tests
- Zebrafish/embryology*
- PubMed
- 25727789 Full text @ J. Appl. Toxicol.
- CTD
- 25727789
Citation
Shen, B., Liu, H.C., Ou, W.B., Eilers, G., Zhou, S.M., Meng, F.G., Li, C.Q., Li, Y.Q. (2015) Toxicity induced by Basic Violet 14, Direct Red 28 and Acid Red 26 in zebrafish larvae. Journal of applied toxicology : JAT. 35(12):1473-80.
Abstract
Basic Violet 14, Direct Red 28 and Acid Red 26 are classified as carcinogenic dyes in the European textile ecology standard, despite insufficient toxicity data. In this study, the toxicity of these dyes was assessed in a zebrafish model, and the underlying toxic mechanisms were investigated. Basic Violet 14 and Direct Red 28 showed acute toxicity with a LC50 value at 60.63 and 476.84 µg ml(-1) , respectively, whereas the LC50 of Acid Red 26 was between 2500 and 2800 µg ml(-1) . Treatment with Basic Violet 14, Direct Red 28 and Acid Red 26 resulted in common developmental abnormalities including delayed yolk sac absorption and swimming bladder deflation. Hepatotoxicity was observed in zebrafish treated with Basic Violet 14, and cardiovascular toxicity was found in zebrafish treated with Acid Red 26 at concentrations higher than 2500 µg ml(-1) . Basic Violet 14 also caused significant up-regulation of GCLC gene expression in a dose-dependent manner whereas Acid Red 26 induced significant up-regulation of NKX2.5 and down-regulation of GATA4 at a high concentration in a dose-dependent manner. These results suggest that Basic Violet 14, Direct Red 28 and Acid Red 26 induce developmental and organ-specific toxicity, and oxidative stress may play a role in the hepatotoxicity of Basic Violet 14, the suppressed GATA4 expression may have a relation to the cardiovascular toxicity of Acid Red 26.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping