PUBLICATION

Developmental regulation of apical endocytosis controls epithelial patterning in vertebrate tubular organs

Authors
Rodríguez-Fraticelli, A.E., Bagwell, J., Bosch-Fortea, M., Boncompain, G., Reglero-Real, N., García-León, M.J., Andrés, G., Toribio, M.L., Alonso, M.A., Millán, J., Perez, F., Bagnat, M., Martín-Belmonte, F.
ID
ZDB-PUB-150224-2
Date
2015
Source
Nature cell biology   17(3): 241-50 (Journal)
Registered Authors
Bagnat, Michel
Keywords
none
MeSH Terms
  • Adaptor Proteins, Vesicular Transport/genetics
  • Adaptor Proteins, Vesicular Transport/metabolism
  • Animals
  • Cell Differentiation
  • Cell Line
  • Cell Polarity
  • Cell Proliferation
  • Embryo, Nonmammalian
  • Endocytosis
  • Endosomes/metabolism*
  • Endosomes/ultrastructure
  • Epithelial Cells/metabolism*
  • Epithelial Cells/ultrastructure
  • Epithelium/metabolism*
  • Epithelium/ultrastructure
  • Gene Expression Regulation, Developmental*
  • Kidney Tubules/metabolism
  • Kidney Tubules/ultrastructure
  • Lysosomes/metabolism*
  • Lysosomes/ultrastructure
  • Mice
  • Myelin and Lymphocyte-Associated Proteolipid Proteins/genetics
  • Myelin and Lymphocyte-Associated Proteolipid Proteins/metabolism
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Receptors, Notch/genetics
  • Receptors, Notch/metabolism
  • SNARE Proteins/genetics
  • SNARE Proteins/metabolism
  • Signal Transduction
  • Zebrafish
PubMed
25706235 Full text @ Nat. Cell Biol.
Abstract
Epithelial organs develop through tightly coordinated events of cell proliferation and differentiation in which endocytosis plays a major role. Despite recent advances, how endocytosis regulates the development of vertebrate organs is still unknown. Here we describe a mechanism that facilitates the apical availability of endosomal SNARE receptors for epithelial morphogenesis through the developmental upregulation of plasmolipin (pllp) in a highly endocytic segment of the zebrafish posterior midgut. The protein PLLP (Pllp in fish) recruits the clathrin adaptor EpsinR to sort the SNARE machinery of the endolysosomal pathway into the subapical compartment, which is a switch for polarized endocytosis. Furthermore, PLLP expression induces apical Crumbs internalization and the activation of the Notch signalling pathway, both crucial steps in the acquisition of cell polarity and differentiation of epithelial cells. We thus postulate that differential apical endosomal SNARE sorting is a mechanism that regulates epithelial patterning.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes