PUBLICATION
Germ cell-specific expression of dead end (dnd) in rare minnow (Gobiocypris rarus)
- Authors
- Duan, J., Feng, G., Chang, P., Zhang, X., Zhou, Q., Zhong, X., Qi, C., Xie, S., Zhao, H.
- ID
- ZDB-PUB-150211-20
- Date
- 2015
- Source
- Fish physiology and biochemistry 41(2): 561-71 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- DNA Primers/genetics
- Molecular Sequence Data
- Reverse Transcriptase Polymerase Chain Reaction/veterinary
- Cyprinidae/embryology*
- Cyprinidae/genetics*
- Cyprinidae/metabolism
- Germ Cells/physiology*
- Sequence Analysis, DNA/veterinary
- Cloning, Molecular
- Gene Expression Regulation, Developmental/physiology*
- Computational Biology
- Base Sequence
- Models, Genetic
- Cluster Analysis
- RNA-Binding Proteins/genetics
- RNA-Binding Proteins/metabolism*
- Amino Acid Sequence
- Likelihood Functions
- Gene Expression Profiling/veterinary
- Sequence Alignment
- Zebrafish Proteins/genetics
- Phylogeny
- In Situ Hybridization
- PubMed
- 25663436 Full text @ Fish Physiol. Biochem.
Citation
Duan, J., Feng, G., Chang, P., Zhang, X., Zhou, Q., Zhong, X., Qi, C., Xie, S., Zhao, H. (2015) Germ cell-specific expression of dead end (dnd) in rare minnow (Gobiocypris rarus). Fish physiology and biochemistry. 41(2):561-71.
Abstract
Rare minnow (Gobiocypris rarus) is an emerging model fish in China, and the development of its gonads is still elusive. Germ cell-specific genes are conserved in animals. Dead end (Dnd) was first documented as a germ granule component in zebrafish. Here, we report the cloning and expression profile of dnd in rare minnow. RT-PCR results showed that dnd is expressed specifically in the gonads of both sexes, is maternal in origin and is expressed continuously during embryogenesis. Dnd mRNA could be detected exclusively in the germ cells of the testis and ovary. Temporal expression of dnd mRNA is similar to that of vasa and dnd in zebrafish during embryogenesis. Taken together, dnd mRNA is restricted to the germ cells of rare minnow.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping